Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis

  • Fangjun Wang

Part of the Springer Theses book series (Springer Theses)

About this book


In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity, and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.


Isotope Dimethylation Labeling Mass Spectrometry Multidimensional Chromatography Separation Polymer-Based Hydrophobic Monolithic Column Protein Phosphorylation Proteome Analysis Quantitative Proteomics Separation of Complex Peptide Mixtures Stable Isotope Labeling Strong Cation-Exchange Monolithic Column

Authors and affiliations

  • Fangjun Wang
    • 1
  1. 1.Dalian Institute of Chemical PhysicsChinese Academy of ScienceDalianPeople's Republic of China

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2014
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-3-642-42007-8
  • Online ISBN 978-3-642-42008-5
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
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