© 2013

Photophysics of Ionic Biochromophores

  • Steen Brøndsted Nielsen
  • Jean Ann Wyer

Part of the Physical Chemistry in Action book series (PCIA)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Steen Brøndsted Nielsen, Jean Ann Wyer
    Pages 1-10
  3. Steen Brøndsted Nielsen
    Pages 11-19
  4. Jean Ann Wyer
    Pages 21-44
  5. Marius Wanko, Angel Rubio
    Pages 45-65
  6. Steen Brøndsted Nielsen
    Pages 105-116
  7. Jean Ann Wyer, Steen Brøndsted Nielsen
    Pages 117-139
  8. Rodolphe Antoine, Philippe Dugourd
    Pages 141-153
  9. Claude Dedonder, Géraldine Féraud, Christophe Jouvet
    Pages 155-180
  10. Thomas Schlathölter, Ronnie Hoekstra
    Pages 209-226
  11. Back Matter
    Pages 227-230

About this book


This book provides a concise overview of the photophysics and spectroscopy of biochromophore ions. The book "Photophysics of Ionic Biochromophores" summarizes important recent advances in the spectroscopy of isolated biomolecular ions in vacuo, which has within the last decade become a highly active research field. Advanced instrumental apparatus and the steady increase in more and more powerful computers have made this development possible, both for experimentalists and theoreticians. Applied techniques described here include absorption and fluorescence spectroscopy, which are excellent indicators of environmental effects and can thus shed light on the intrinsic electronic structures of ions without perturbations from e.g. water molecules, counter ions, nearby charges, and polar amino acid residues. When compared with spectra of the chromophores in their natural environment, such spectra allow to identify possible perturbations. At the same time gas-phase spectra provide important benchmarks for quantum chemistry calculations of electronically excited states.

This volume focuses on biological systems from protein biochromophores, e.g. the protonated Schiff-base retinal responsible for vision, and individual aromatic amino acids to peptides and whole proteins, studied using visible, ultraviolet and vacuum ultraviolet light. Work on DNA nucleotides and strands that are amenable to mass spectrometric studies because of the negatively charged sugarphosphate backbone are also presented. DNA strands represent an example of the interplay between multiple chromophores, which is even harder to model correctly than just single chromophores due to spatially extended excited states and weak coupling terms. The experimental techniques used to measure spectra and commonly used theoretical methods are described with a discussion on limitations and advantages. The volume includes an updated status of the field and interesting future directions such as cold ion spectroscopy.


Absorption and Fluorescence Spectroscopy Amino Acid, Peptide and Protein Ions Biochromophore Ions Chromophore Interactions DNA Nucleotides and Strands Excited-state Dynamics Photobiophysics

Editors and affiliations

  • Steen Brøndsted Nielsen
    • 1
  • Jean Ann Wyer
    • 2
  1. 1.Department of Physics and AstronomyAarhus University Faculty of ScienceAarhusDenmark
  2. 2.Department of Physics and AstronomyAarhus University Faculty of ScienceAarhus CDenmark

Bibliographic information

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