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© 2014

Gigaseal Formation in Patch Clamping

With Applications of Nanotechnology

  • Explains the mechanisms of gigaseal formation to the latest level of discoveries

  • Provides practical techniques, which enable frequent formation of high resistance seals

  • Shares authors´ recent practice in acquiring gigaseal formation using micropipettes

Book

Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Majid Malboubi, Kyle Jiang
    Pages 1-2
  3. Majid Malboubi, Kyle Jiang
    Pages 3-16
  4. Majid Malboubi, Kyle Jiang
    Pages 17-28
  5. Majid Malboubi, Kyle Jiang
    Pages 29-47
  6. Majid Malboubi, Kyle Jiang
    Pages 49-59
  7. Majid Malboubi, Kyle Jiang
    Pages 61-73

About this book

Introduction

This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.

Keywords

Gigaseal Formation using micropipettes High Resistance Seals High throughput screening Hydrophilicity Nanotechnology Patch Clamp Patch clamping Surface Roughness Tip Size

Authors and affiliations

  1. 1.London Centre for NanotechnologyLondonUnited Kingdom
  2. 2.School of Mechanical EngineeringThe University of BirminghamBirminghamUnited Kingdom

Bibliographic information

  • Book Title Gigaseal Formation in Patch Clamping
  • Book Subtitle With Applications of Nanotechnology
  • Authors Majid Malboubi
    Kyle Jiang
  • Series Title SpringerBriefs in Applied Sciences and Technology
  • Series Abbreviated Title SpringerBriefs in Applied Sciences
  • DOI https://doi.org/10.1007/978-3-642-39128-6
  • Copyright Information The Author(s) 2014
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Engineering Engineering (R0)
  • Softcover ISBN 978-3-642-39127-9
  • eBook ISBN 978-3-642-39128-6
  • Series ISSN 2191-530X
  • Series E-ISSN 2191-5318
  • Edition Number 1
  • Number of Pages XI, 94
  • Number of Illustrations 16 b/w illustrations, 65 illustrations in colour
  • Topics Nanotechnology and Microengineering
    Biomedical Engineering and Bioengineering
  • Buy this book on publisher's site
Industry Sectors
Electronics
Engineering
Oil, Gas & Geosciences

Reviews

“This is a very good book on patch clamp protocol with a focus on the engineering and physics of pipettes. A description on tuning for bore diameter and various approaches to the culture dishes is detailed. … This is a reliable resource for use in the physiology labs. Physiologists, students, postdocs, & senior investigators will find this very helpful.” (Joseph J. Grenier, Amazon.com, September, 2015)