© 2013

Analyzing Microbes

Manual of Molecular Biology Techniques

  • Dilip Kumar Arora
  • Surajit Das
  • Mesapogu Sukumar


  • Provides step-by-step instructions for immediate use in the laboratory

  • With flow charts of all experiments

  • Includes suppliers list and recipes of solutions and media


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, Dilip K. Arora
    Pages 1-16
  3. Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, Dilip K. Arora
    Pages 31-36
  4. Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, Dilip K. Arora
    Pages 73-91
  5. Bandamaravuri Kishore Babu, T. Kiran Babu, Rajan Sharma
    Pages 93-97
  6. Bhim Pratap Singh, Ratul Saikia
    Pages 105-112
  7. Hirak Ranjan Dash, Neelam Mangwani, Surajit Das
    Pages 113-122
  8. Kamlesh K. Meena, Manish Kumar, D. P. Singh
    Pages 123-134
  9. Sudheer Kumar, Prem L. Kashyap, Ruchi Singh, Alok K. Srivastava
    Pages 135-152
  10. Jyoti Verma, Sangeeta Saxena, Sunil G. Babu
    Pages 169-186
  11. Thangamani Rajesh, Jeyaprakash Rajendhran, Paramasamy Gunasekaran
    Pages 187-208
  12. Suchi Smita, Krishna P. Singh, Bashir A. Akhoon, Shishir K. Gupta, Shailendra K. Gupta
    Pages 209-243
  13. Surajit Das, Hirak Ranjan Dash
    Pages 245-260
  14. Hirak Ranjan Dash, Surajit Das
    Pages 281-290
  15. Bandamaravuri Kishore Babu, Anu Sharma, Hari Kishan Sudini
    Pages 291-302

About this book


This Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to successfully apply the procedures are included, as well as recommendations regarding materials and suppliers. In addition to the practical protocols, important background information and representative results of experiments using the described methods are presented. Researchers in all areas applying microbial systems, such as in molecular biology, genetics, pathology, and agricultural research will find this work of great value.


ELISA Fingerprinting Gel electrophoresis Metagenomic libraries Microarrays Restriction enzymes

Editors and affiliations

  • Dilip Kumar Arora
    • 1
  • Surajit Das
    • 2
  • Mesapogu Sukumar
    • 3
  1. 1.National Bureau of Agriculturally ImportMaunath BhanjanIndia
  2. 2., Dep. Life SciencesNational Institute of TechnologyRourkelaIndia
  3. 3.National Bureau of Agriculturally ImportMaunath BhanjanIndia

Bibliographic information

  • Book Title Analyzing Microbes
  • Book Subtitle Manual of Molecular Biology Techniques
  • Editors Dilip Kumar Arora
    Surajit Das
    Mesapogu Sukumar
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2013
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-3-642-34409-1
  • Softcover ISBN 978-3-662-50967-8
  • eBook ISBN 978-3-642-34410-7
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages XI, 352
  • Number of Illustrations 18 b/w illustrations, 62 illustrations in colour
  • Topics Microbiology
    Medical Microbiology
    Biochemistry, general
  • Buy this book on publisher's site
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From the reviews:

“The Analyzing Microbes­—Manual of Molecular Biology Techniques is indeed a practical guide to the application of various molecular biology techniques in general microbiological research, with special emphasis in/on biotechnology and environmental microbiology. … All chapters are written in a concise step-by-step algorithm and are easy to follow during reading or bench working. … In my opinion, the aim of this book … to produce a self-contained laboratory manual which will be useful to both experienced practitioners and beginners in the field, is fully accomplished.” (Ioan I. Ardelean, Romanian Journal of Biochemistry, Vol. 50 (1), 2013)