© 2013

Fluorescent Methods to Study Biological Membranes

  • Yves Mély
  • Guy Duportail

Part of the Springer Series on Fluorescence book series (SS FLUOR, volume 13)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Sourav Haldar, Amitabha Chattopadhyay
    Pages 37-50
  3. Galyna Gorbenko, Paavo K. J. Kinnunen
    Pages 115-140
  4. Sarka Pokorna, Agnieszka Olżyńska, Piotr Jurkiewicz, Martin Hof
    Pages 141-159
  5. Claire E. Butler, Guy Wheeler, Jeremy Graham, Kevin M. Tyler
    Pages 163-184
  6. Daniel Wüstner, Frederik W. Lund, Lukasz M. Solanko
    Pages 185-213
  7. Martin T. Stöckl, Ranieri Bizzarri, Vinod Subramaniam
    Pages 215-240
  8. Monika Zelman-Femiak, Yamunadevi Subburaj, Ana J. García-Sáez
    Pages 241-269
  9. Vincent Rouger, Cyrille Billaudeau, Tomasz Trombik, Sébastien Mailfert, Yannick Hamon, Hai-Tao He et al.
    Pages 271-289
  10. Christian Eggeling
    Pages 291-309
  11. Sophie Brasselet, Patrick Ferrand, Alla Kress, Xiao Wang, Hubert Ranchon, Alicja Gasecka
    Pages 311-337
  12. Thomas S. van Zanten, Carlo Manzo, Maria F. Garcia-Parajo
    Pages 339-363
  13. Martin Cottet, Orestis Faklaris, Eric Trinquet, Jean-Philippe Pin, Thierry Durroux
    Pages 389-415
  14. Felix Neugart, Darius Widera, Barbara Kaltschmidt, Christian Kaltschmidt, Mike Heilemann
    Pages 439-455
  15. Back Matter
    Pages 479-485

About this book


Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.


advanced fluorescence microscopy lipid domains membrane organization and dynamics membrane probes membrane proteins membrane receptors

Editors and affiliations

  • Yves Mély
    • 1
  • Guy Duportail
    • 2
  1. 1.Faculté de Pharmacie, Laboratoire de BiophotoniqueUniversité de StrasbourgIllkirchFrance
  2. 2.Faculté de Pharmacie, Laboratoire de Biophotonique etUniversité de StrasbourgIllkirchFrance

Bibliographic information

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From the reviews:

“The book covers all major applications of fluorescence to membrane studies. … The book is addressed to scientists and PhD students using fluorescence methods to study biological membranes and membrane proteins. This is a highly interdisciplinary field, and the book will therefore be of interest to biophysicists, physical chemists, and cellular biologists … . broad overview of different techniques provided by the book is also a good starting point for newcomers to the field. … a valuable resource for everyone studying membranes and membrane proteins.” (Lorenzo Stella, Analytical and Bioanalytical Chemistry, May, 2013)