© 2011

Advanced Protocols for Animal Transgenesis

An ISTT Manual

  • Shirley Pease
  • Thomas L. Saunders


  • Provides step-by-step instructions and helpful troubleshooting notes

  • Published in cooperation with the International Society for Transgenic Technology (ISTT)

  • With contributions by leading scientists


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Eduardo Moltó, Cristina Vicente-García, Lluis Montoliu
    Pages 43-56
  3. Thomas J. Fielder, Lluis Montoliu
    Pages 81-97
  4. Katja Becker, Boris Jerchow
    Pages 99-115
  5. Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal, Anne-Laure Iscache, Ignacio Anegon
    Pages 117-135
  6. Almudena Fernández, Diego Muñoz, Lluis Montoliu
    Pages 137-158
  7. Michael G. Zeidler, Margaret L. Van Keuren, Thomas L. Saunders
    Pages 159-179
  8. Aron Geurts, Darius Balciunas, Lajos Mates
    Pages 213-236
  9. Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson, F. Kent Hamra
    Pages 237-266
  10. Sayaka Wakayama, Nguyen Van Thuan, Teruhiko Wakayama
    Pages 267-289
  11. Elizabeth D. Hughes, Thomas L. Saunders
    Pages 291-325
  12. Wojtek Auerbach, Anna B. Auerbach
    Pages 327-356
  13. Marina Gertsenstein
    Pages 357-375
  14. Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara, Marina Gertsenstein
    Pages 377-430
  15. Kristina Nagy, Jennifer Nichols
    Pages 431-455
  16. Ping Li, Eric N. Schulze, Chang Tong, Qi-Long Ying
    Pages 457-475

About this book


This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.


Embryonic stem cells Knocked out genes Nuclear gene transfer Pluripotent cells Transgenic animals Transposable elements YACs

Editors and affiliations

  • Shirley Pease
    • 1
  • Thomas L. Saunders
    • 2
  1. 1.Div. BiologyCalifornia Institute of TechnologyPasadenaUSA
  2. 2.Transgenic Animal Model CoreUniversity of MichiganAnn ArborUSA

Bibliographic information

  • Book Title Advanced Protocols for Animal Transgenesis
  • Book Subtitle An ISTT Manual
  • Editors Shirley Pease
    Thomas L. Saunders
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2011
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-3-642-20791-4
  • Softcover ISBN 978-3-662-51943-1
  • eBook ISBN 978-3-642-20792-1
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages XV, 669
  • Number of Illustrations 145 b/w illustrations, 65 illustrations in colour
  • Topics Animal Genetics and Genomics
  • Buy this book on publisher's site
Industry Sectors