Restriction Endonucleases

  • Alfred M. Pingoud

Part of the Nucleic Acids and Molecular Biology book series (NUCLEIC, volume 14)

Table of contents

  1. Front Matter
    Pages I-XXVI
  2. R. J. Roberts, M. Belfort, T. Bestor, A. S. Bhagwat, T. A. Bickle, J. Bitinaite et al.
    Pages 1-18
  3. F. K. Winkler, A. E. Prota
    Pages 179-214
  4. É. Scheuring Vanamee, H. Viadiu, C. M. Lukacs, A. K. Aggarwal
    Pages 215-236
  5. V. Siksnys, S. Grazulis, R. Huber
    Pages 237-259
  6. N. Sidorova, D. C. Rau
    Pages 319-337
  7. J. Alves, P. Vennekohl
    Pages 393-411
  8. K. Kandavelou, M. Mani, S. Durai, S. Chandrasegaran
    Pages 413-434
  9. Back Matter
    Pages 435-443

About this book


Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.

The present book deals with all aspects of restriction endonucleases including nomenclature, diversity, evolution, genetics, structure and function, mechanism of target site location and DNA recognition, enzymology, protein design, and provides a description of the history of the discovery of and the research on restriction enzymes.


ATP DNA DNA-Erkennung DNA-Spaltung Restriktionsenzym bacteria base enzyme enzymes evolution genetics nucleic acid protein protein engineering replication

Editors and affiliations

  • Alfred M. Pingoud
    • 1
  1. 1.Institute for BiochemistryJustus-Liebig-UniversityGiessenGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2004
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-62324-0
  • Online ISBN 978-3-642-18851-0
  • Series Print ISSN 0933-1891
  • Series Online ISSN 1869-2486
  • Buy this book on publisher's site
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