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Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging

  • Anna Siri Luthman

Part of the Springer Theses book series (Springer Theses)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Anna Siri Luthman
    Pages 1-7
  3. Anna Siri Luthman
    Pages 51-85
  4. Anna Siri Luthman
    Pages 87-130
  5. Anna Siri Luthman
    Pages 131-136
  6. Back Matter
    Pages 137-137

About this book

Introduction

This book describes the design, development, characterisation and application of two novel fluorescence imaging instruments based on spectrally resolved detector arrays (SRDAs). The simplest SRDA is the standard colour camera, which integrates a Bayer filter array of red, green and blue colour filters to replicate the colour sensing capability of the human eye. The SRDAs used in this book contain many more colours, ranging from 16 to over 100 colour channels. Using these compact, robust and low-cost detectors for biomedical applications opens new avenues of exploration that were not possible before, in particular, the use of spectral imaging in endoscopy. The work presented shows for the first time that not only can this new type of camera be used for fluorescence imaging, but also that it is able to resolve signals from up to 7 different dyes – a level of multiplexing not previously achieved in tissue with such compact and robust equipment. Furthermore, it reports the application of a bimodal endoscope performing both reflectance and fluorescence imaging using these cameras in an ex vivo pig oesophagus model. This is the first book to demonstrate the potential of SRDAs for fluorescence imaging in biomedicine, opening up a new multidisciplinary research direction.

Keywords

Hyperspectral Imaging Multispectral Imaging Spectrally Resolving Detector Arrays Camera Calibration Endoscopy In Vivo Imaging

Authors and affiliations

  • Anna Siri Luthman
    • 1
  1. 1.Department of PhysicsUniversity of CambridgeCambridgeUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-319-98255-7
  • Copyright Information Springer Nature Switzerland AG 2018
  • Publisher Name Springer, Cham
  • eBook Packages Physics and Astronomy
  • Print ISBN 978-3-319-98254-0
  • Online ISBN 978-3-319-98255-7
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
  • Buy this book on publisher's site
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