© 2018

Basic Confocal Microscopy

  • W. Gray (Jay) Jerome
  • Robert L. Price
  • Fully updated and expanded to reflect developments in the field yet still concise and comprehensive

  • Covers the basics needed to design, implement, and interpret the results of biological experiments based on confocal microscopy, as well as resources for continued learning

  • All chapters are written by well-recognized experts in the field

  • While not a traditional textbook, this is the perfect companion volume for the Confocal Microscopy Workshops presented regularly by the authors and others


Table of contents

  1. Front Matter
    Pages i-xi
  2. Robert L. Price, W. Gray (Jay) Jerome
    Pages 1-20
  3. W. Gray (Jay) Jerome
    Pages 21-36
  4. W. Gray (Jay) Jerome, Robert L. Price
    Pages 37-71
  5. W. Gray (Jay) Jerome, John Fuseler, Caleb A. Padgett, Robert L. Price
    Pages 73-97
  6. R. M. Albrecht, J. A. Oliver
    Pages 99-134
  7. W. Gray (Jay) Jerome
    Pages 135-153
  8. W. Gray (Jay) Jerome
    Pages 155-186
  9. John Fuseler, W. Gray (Jay) Jerome, Robert L. Price
    Pages 187-213
  10. Amy E. Rowley, Anna M. Harper, Robert L. Price
    Pages 215-277
  11. Thomas C. Trusk
    Pages 279-307
  12. Jesse Aaron, Teng-Leong Chew
    Pages 309-333
  13. W. Gray (Jay) Jerome, Robert L. Price
    Pages 335-342
  14. Back Matter
    Pages 343-368

About this book


Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.  


fluorescence multiphoton 3d reconstruction spectral imaging deconvolution

Editors and affiliations

  • W. Gray (Jay) Jerome
    • 1
  • Robert L. Price
    • 2
  1. 1.Department of Pathology, Microbiology and ImmunologyVanderbilt University School of MedicineNashvilleUSA
  2. 2.Department of Cell Biology and AnatomyUniversity of South Carolina School of MedicineColumbiaUSA

About the editors

Dr. W. Gray (Jay) Jerome is an Associate Professor of Pathology, Microbiology and Immunology at Vanderbilt University School of Medicine. He has published extensively on microscopy of biological material. His work involves correlating microscopic structural information with biochemical, physiological, and molecular genetic data to provide a comprehensive picture of structure-function relationships. Dr. Jerome is a Fellow of the Microscopy Society of America, a Fellow of the American Association for the Advancement of Science, a Fellow of the American Heart Association and a Past-President of the Microscopy Society of America.


Dr. Robert L. Price is a Research Professor in Cell Biology and Anatomy at the University of South Carolina School of Medicine and Director of the Instrumentation Resource Facility. He has been active in biomedical imaging using confocal microscopy since 1990 and along with Dr. Jerome has taught numerous Confocal Workshops in the United States and Australia.  Dr. Price has received several awards from the University of South Carolina and the Microscopy Society of America, is a Fellow of the Microscopy Society of America, and currently President of the Microscopy Society of America. 

Bibliographic information