© 2018

Biotechnological Applications of Plant Proteolytic Enzymes

  • María Gabriela Guevara
  • Gustavo Raúl Daleo


  • Raises awareness of the need for low-toxicity and environmentally friendly enzymes extracted from natural resources

  • Offers insights into the biotechnological use of proteases

  • Provides a comprehensive and up-to-date review of plant proteases and their biotechnological applications


Table of contents

  1. Front Matter
    Pages i-ix
  2. D’Ipólito Sebastián, María Gabriela Guevara, Tito Florencia Rocío, Tonón Claudia Virginia
    Pages 1-19
  3. Mazorra-Manzano Miguel A., Moreno-Hernández Jesús M., Ramírez-Suarez Juan C.
    Pages 21-41
  4. Thirawat Tantamacharik, Alan Carne, Dominic Agyei, John Birch, Alaa El-Din Ahmed Bekhit
    Pages 43-67
  5. Sonia Barberis, Mauricio Adaro, Anabella Origone, Grisel Bersi, Fanny Guzmán, Andrés Illanes
    Pages 69-106
  6. Carlos E. Salas, Dalton Dittz, Maria-Jose Torres
    Pages 107-127
  7. Alfonso Pepe, María Gabriela Guevara, Florencia Rocío Tito
    Pages 129-141
  8. Back Matter
    Pages 143-146

About this book


This book offers an overview of the diverse fields application of proteases (also termed proteolytic enzymes or proteinases), including food science and technology, pharmaceutical industries, and detergent manufacturing, reviewing the advances in the biotechnological application plant proteolytic enzymes over the last decade. In recent years, they have been the focus of renewed attention from the pharmaceutical and biotechnology industries, not only because of their activity on a wide variety of proteins but also because they are active over a range of temperatures and pHs. The main audience of this book are researchers working with plant proteases but also professionals from several industry segments such as food production and pharmaceutical companies.


Plant proteases Plant proteolytic enzymes Plant proteases biotechnology Plant enyzmes Vegetal proteolytic enzymes biotechnology

Editors and affiliations

  1. 1.Biological Research InstituteUniversity of Mar del PlataMar del PlataArgentina
  2. 2.Biological Research InstituteUniversity of Mar del PlataMar del PlataArgentina

About the editors

Dr. María G. Guevara graduated in Biology (MSc. 1996) at the University of Mar del Plata (UNMDP), Argentina. She obtained her PhD in 2003 in Plant Biochemistry and Molecular Biology at the University of Mar del Plata including a short research stay at the Center for Neuroscience and Cell Biology of Coimbra (CNC), University of Coimbra, Portugal. From 2001to 2006 she investigated the role of typical plant aspartic proteases in plant defense against pathogens, which was first research to report the bifunctional activity (proteolytic/ antimicrobial) of these enzymes. Additionally, her publications have contributed to elucidating the mechanism of action of these enzymes and their domains in vitro and during plant pathogen interactions. Gabriela Guevara is currently the leader of the Plant Proteases Laboratory at the Biological Research Institute (IIB) of the National Scientific and Technical Research Council (CONICET) and University of Mar del Plata. Her research activities are mainly focused on proteolytic enzymes in both basic and applied research in biotechnology, in areas as diverse as health, plant biology and food applications.

Dr. Gustavo R. Daleo joined the IIB in 1985, as head of the Plant Biochemistry Laboratory. He is a professor of Biochemistry at the Faculty of Exact and Natural Sciences, National University of Mar del Plata. Professor Daleo received his Licenciado degree and his PhD in Chemical Sciences from the Buenos Aires University in 1976. He was a postdoctoral fellow at the Biology Department, Bariloche Foundation (1976-1977) and at the Department of Biochemistry, Michigan State University (1977-1979). In 1980 he joined the University of Mar del Plata, and at the IIB, where he introduced biochemical and molecular mechanisms in plant defense against pathogens and abiotic stress as a field of study. His research group has produced several publications that contribute to the formulation of strategies for integrated crop management and to the use of plant proteins, peptides and other compounds as antimicrobial and therapeutic agents.

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