Multi-Parametric Live Cell Microscopy of 3D Tissue Models

  • Ruslan I. Dmitriev

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1035)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction: 3D Tissue Models, Methodology and Toolkit

    1. Front Matter
      Pages 1-1
    2. Katie Bardsley, Anthony J. Deegan, Alicia El Haj, Ying Yang
      Pages 3-18
    3. Alix Le Marois, Klaus Suhling
      Pages 31-48
  3. Manufacturing

    1. Front Matter
      Pages 69-69
    2. Anastasia Shpichka, Anastasia Koroleva, Daria Kuznetsova, Ruslan I. Dmitriev, Peter Timashev
      Pages 71-81
  4. Application Methods and Protocols

    1. Front Matter
      Pages 83-83
    2. Irina A. Okkelman, Tara Foley, Dmitri B. Papkovsky, Ruslan I. Dmitriev
      Pages 85-103
    3. Elena V. Zagaynova, Irina N. Druzhkova, Natalia M. Mishina, Nadezhda I. Ignatova, Varvara V. Dudenkova, Marina V. Shirmanova
      Pages 105-119
    4. Asylkhan Rakymzhan, Helena Radbruch, Raluca A. Niesner
      Pages 135-141
    5. Marina V. Shirmanova, Lubov’ E. Shimolina, Maria M. Lukina, Elena V. Zagaynova, Marina K. Kuimova
      Pages 143-153
    6. Marco Marcello, Rosalie Richards, David Mason, Violaine Sée
      Pages 155-161
    7. Shinji Kajimoto, Mizuki Takeuchi, Takakazu Nakabayashi
      Pages 163-172

About this book


This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal  book for specialists working in tissue engineering and designing novel biomaterial.


3D live cell imaging 3D tissue models Applications Multi-parametric imaging Autofluorescence imaging in cancer Ca2+ imaging neural Multi-parametric imaging cancer Multi-parametric imaging of pH Multi-parametric imaging viscosity cancer Multi-parametric quantitative imaging Quantitative imaging intestinal organoid

Editors and affiliations

  • Ruslan I. Dmitriev
    • 1
  1. 1.Metabolic Imaging Group, School of Biochemistry and Cell BiologyUniversity College CorkCorkIreland

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