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© 2017

Pollen Tip Growth

From Biophysical Aspects to Systems Biology

  • Gerhard Obermeyer
  • José Feijó
Book

Table of contents

  1. Front Matter
    Pages i-viii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Gerhard Obermeyer, José Feijó
      Pages 3-10
  3. Biophysics of Tip Growth

    1. Front Matter
      Pages 11-11
    2. Gerhard Obermeyer
      Pages 13-34
  4. Technical Improvements to Study Tip Growth

  5. Subcellular Processes

    1. Front Matter
      Pages 105-105
    2. Hao Wang, Liwen Jiang
      Pages 107-127
    3. Subramanian Sankaranarayanan, Tetsuya Higashiyama
      Pages 149-166
    4. Sébastjen Schoenaers, Daria Balcerowicz, Kris Vissenberg
      Pages 167-243
  6. Tipomics: Omic Approaches in Tip Growth

    1. Front Matter
      Pages 245-245
    2. Jan Fíla, Lenka Záveská Drábková, Antónia Gibalová, David Honys
      Pages 247-292
    3. Heidi Pertl-Obermeyer
      Pages 293-318
    4. Thomas Nägele, Lena Fragner, Palak Chaturvedi, Arindam Ghatak, Wolfram Weckwerth
      Pages 319-336
  7. Modeling Tip Growth

    1. Front Matter
      Pages 337-337
    2. Robert W. Smith, Christian Fleck
      Pages 339-367
    3. Milenka Van Hemelryck, Roberto Bernal, Enrique Rojas, Jacques Dumais, Jens H. Kroeger
      Pages 369-389
    4. Daniel S. C. Damineli, Maria Teresa Portes, José A. Feijó
      Pages 391-413
  8. Back Matter
    Pages 415-424

About this book

Introduction

This book focuses on the biophysical aspects of tip growth: How do physical parameters like pressure, water potential, electrical fields, or ion currents contribute to and influence this specialized and highly dynamic form of cell growth?

It provides an updated and balanced overview of the current state of knowledge and future research perspectives regarding how pollen tubes’ growth is driven and regulated by molecular interactions underlying the cellular processes. The individual chapters address topics ranging from molecular biophysical concepts to comprehensive omic studies and computational modeling of the tip growth process. In addition, a chapter on root hair cells is included to provide an alternative view on the underlying molecular principles of tip growth in general.

Each chapter provides a summary of cutting-edge techniques, results and experimental data; a statement, hypothesis or conclusion on the importance of the aspect described for tip growth, particularly pollen tip growth; and addresses open gaps in our understanding and potential approaches to remedying them.

The book offers experimental and theoretical solutions to help established researchers and newcomers to the field alike solve the many mysterious behaviors of pollen tube tips.

Keywords

pollen tube tip growth cell mechanics cell growth pollen grain systems biology membrane trafficking membrane proteomics root hair

Editors and affiliations

  • Gerhard Obermeyer
    • 1
  • José Feijó
    • 2
  1. 1.Department of Molecular BiologyUniversity of SalzburgSalzburgAustria
  2. 2.Cell Biology Molecular GeneticsUniversity of MarylandCollege ParkUSA

About the editors

Gerhard Obermeyer studied Biology at the University of Constance, Germany, with majors in membrane and cell biophysics. His Ph.D. thesis at the Technical University of Karlsruhe (Germany) included the quantitative imaging of tip-localised Ca2+ gradients in pollen tubes and first patch-clamp experiments to characterise pollen ion channels. As a post-doc he worked at Wye College (now part of Imperial College, London, UK) on ion channels from guard cells and pollen grains, and continued pollen research at the Institute of Plant Physiology (Univ. Salzburg, Austria) with intermediate fellowships to visit labs at the Univ. Adelaide (Australia), at Univ. Nacional Autonoma de Mexico (Cuernavaca, Mexico) and at Univ. Oxford (UK) to work on symbiosome membranes and intracellular pH measurements, respectively. Finally, he became associate professor at the Univ. Salzburg where he established molecular plant physiology in research and teaching.

Prof Obermeyer’s research focusses mainly on pollen physiology. Growth of pollen tubes through the style tissue is a prerequisite for successful fertilization which guarantees high crop yields for human nutrition. Problems caused by global warming like drought and temperature stress, can disturb pollen function and are studied using several single molecule/cell techniques in combination with -omics approaches to reveal functional protein complexes in the plasma membrane and their role in osmosensing and osmoregulation as well as in tip growth.

 

José Feijó studied Biology in the University of Lisbon, Portugal, specializing in Cell Biology of orchid pollen and a Master in Plant Biotechnology. During his Ph.D. he enlarged his focus into development, progressively introducing electrophysiology and mathematical modelling as routine approaches to the study of pollen tubes. A Fullbright fellowship brought him to Peter Hepler lab in the U. Massachussets in Amherst to further deepening his skills on ion imaging. Return to the University of Lisbon in 1996 marked the beginning of a professorship and independent research career, which from 1999-2013 he ran in parallel with an independent lab in the Gulbenkian Institute for Science. Along this path, he has served as director of the Imaging Unit, organized over a dozen EMBO Practical course on Plant Development and Imaging, acted as curator and was responsible for a number of initiatives to commemorate Darwin’s bicentenary in 2009 and collaborated with numerous educational projects. In late 2013 he moved to the University of Maryland, in College Park, and lives in Washington DC. 

Research in Feijó’s group is focused on the development of integrated models of apical cell growth and morphogenesis, using the pollen tube as a biological model, ion dynamics as an experimental paradigm and theoretical modeling as an integrative tool. The group uses Arabidopsis, lily, tobacco and tomato as model species for higher plants, and the moss Physcomitrella as an evolutionary correlate of apical growth evolution.  On the path to develop models by which ion dynamics choreographies integrate spatial and temporal cues to coordinate cell biology, the group contributed with novel ion channels and sensors involved in pollen tube biology, and their regulation mechanisms. Most results involve a combination of imaging, electrophysiology, genetics and molecular biology. Feijó’s group further pioneered transcriptomics of plant male gametes and its consequences for plant reproduction and improvement, namely at the epigenetics level. These activities were routinely grounded in collaborations with groups on over 12 countries in the 4 continents. 

The authors met at the Sexual Plant Reproduction meeting in Vienna, Austria, 1998, and collaborate and became good friends ever since.

Bibliographic information

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