© 2017

Modern Tools and Techniques to Understand Microbes

  • Ajit Varma
  • Arun Kumar Sharma


  • Demonstrates modern laboratory procedures for analyzing microbes

  • Provides up-to-date techniques for research and teaching

  • Offers novel approaches to identify and characterize microorganisms


Table of contents

  1. Front Matter
    Pages i-xiii
  2. Pankaj Goyal, Diksha Bhola, Ajit Varma
    Pages 1-23
  3. Gamze Nur Kara, Mustafa Guzel, Bulent Kabak
    Pages 25-43
  4. Diksha Bhola, Varma Ajit
    Pages 45-63
  5. Avinash P. Ingle, Dnyaneshwar Rathod, Marian Brestic, Hazem M. Kalaji, Mahendra Rai
    Pages 65-80
  6. Ramesh N. Pudake, Maya Kumari, Binod Bihari Sahu, Eram Sultan
    Pages 81-102
  7. Neeraj Shrivastava, Vivek Kumar, Manoj Kumar, Binggan Lou, Ajit Varma, Shwet Kamal
    Pages 103-114
  8. Devendra K. Choudhary, Ajit Varma
    Pages 115-122
  9. Jwad E. Mahdi, Lynette K. Abbott, Natasha Pauli, Zakaria M. Solaiman
    Pages 123-134
  10. Antonia Susca, Antonio Moretti, Antonio F. Logrieco
    Pages 135-148
  11. Cruz Cristina, Catarina Gouveia, Teresa Dias, Ajit Varma, Olubukola O. Babalola
    Pages 149-158
  12. Abdul Haseeb Shah, Sudhanshu Shukla, Rajendra Prasad
    Pages 183-207
  13. Pankaj Goyal, Nim J. Singh, Rupesh Kumar Basniwal, Manoj Kumar, Vivek Kumar, Narendra Tuteja et al.
    Pages 209-228
  14. Smriti Shrivastava, Pratyoosh Shukla, Kunal Mukhopadhyay, Ajit Varma
    Pages 229-235
  15. Stefanie P. Glaeser, Ibrahim Alabid, Huijuan Guo, Neelendra Kumar, Jafargholi Imani, Peter Kämpfer et al.
    Pages 237-250
  16. Vivek Kumar, Priyanku Teotia, Narnedra Tuteja, Ram Prasad, Pankaj Goyal, Ajit Varma et al.
    Pages 251-257
  17. Priyanka Sharma, Hemesh Joshi, MZ Abdin, Amit C Kharkwal, Ajit Varma
    Pages 259-271
  18. Manoj Kumar, Neeraj Shrivastava, Priyanku Teotia, Pankaj Goyal, Ajit Varma, Shivesh Sharma et al.
    Pages 273-283

About this book


This book provides essential molecular techniques and protocols for analyzing microbes that are useful for developing novel bio-chemicals, such as medicines, biofuels, and plant protection substances.

The topics and techniques covered include: microbial diversity and composition; microorganisms in the food industry; mass cultivation of sebacinales; host-microbe interaction; targeted gene disruption; function-based metagenomics to reveal the rhizosphere microbiome; mycotoxin biosynthetic pathways; legume-rhizobium symbioses; multidrug transporters of yeast; drug-resistant bacteria; the fungal endophyte piriformospora indica; medicinal plants; arbuscular mycorrhizal fungi; biosurfactants in microbial enhanced oil recovery; and biocontrol of the soybean cyst nematode with root endophytic fungi; as well as microbe-mediated drought tolerance in plants.

• Demonstrates modern laboratory procedures for analyzing microbes

• Provides up-to-date techniques for research and teaching

• Offers novel approaches to identify and characterize microorganisms


Biofertilizer Drug resistant bacteria and yeasts Flow Cytometry Host-microbe interaction Mycotoxin Site Directed Mutagenesis Soil rhizosphere

Editors and affiliations

  • Ajit Varma
    • 1
  • Arun Kumar Sharma
    • 2
  1. 1.Amity Institute of Microbial TechnologyAmity University Uttar PradeshNoidaIndia
  2. 2.ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM)Kusmaur, Mau Nath BhanjanIndia

About the editors

Prof. Dr. Ajit Varma, M.Sc. Ph.D. is Distinguished Scientist and Professor of Eminence at Amity University Uttar Pradesh, Noida (India). He is recipient of Alexander Von Humboldt Foundation Fellowship (Germany).  Dr. Varma research interest is Plant-Microbe Interactions and Mycorrhizal fungi. He has discovered cultivable mycorrhiza and named it Piriformospora indica. As on today, he has authored and edited 78 books and published 314 research articles in International Journal of repute. Dr. Varma is the Editor -in -Chief of Soil Biology Series being published by Springer Verlag, Germany.

Dr Arun Kumar Sharma did his M.Sc. and Ph.D. from Jammu University, Jammu, J&K, in the discipline of Botany, specializing in Mycology and Plant Pathology.  He joined the Agricultural Research Service (ARS) under the Indian Council of Agricultural Research during 1978 and has the research experience of over 37 years in ICAR in various capacities. He also worked as Plant Pathologist under Food and Agriculture Organization (FAO) of the United Nations in South America for a couple of years. Dr.  Sharma has worked as Director, ICAR-National Bureau of Agriculturally Important Microorganisms (Department of Agricultural Research and Education, DARE, Govt. of India). ICAR–NBAIM is the Repository of the agriculturally important microorganisms, designated by the National Biodiversity Authority of India. It holds more than 6500 microbial cultures in its culture collection. He has a vast experience of working on rusts, Karnal Bunt and other major diseases of wheat, pest problems of rice, IPM, pest survey–surveillance, Human Resource Development, agriculturally important microorganisms and other allied sectors.  Dr. Sharma has a great interest in Agriculturally Important Micro-organisms, especially in the utilization of microorganisms in crop health management and other allied areas.

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