© 2016

The Next Generation in Membrane Protein Structure Determination

  • Isabel Moraes


  • Gives novel approaches to structure determination of membrane proteins

  • Provides good coverage on how membrane proteins interact with their surrounding environment

  • Is a useful guide not only for students, but also for experienced scientists who need to deal with very small and problematic protein crystals


Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 922)

Table of contents

  1. Front Matter
    Pages i-x
  2. Louise E. Bird, Joanne E. Nettleship, Valtteri Järvinen, Heather Rada, Anil Verma, Raymond J. Owens
    Pages 1-11
  3. Anandhi Anandan, Alice Vrielink
    Pages 13-28
  4. Sundaresan Rajesh, Michael Overduin, Boyan B. Bonev
    Pages 29-42
  5. Joanne L. Parker, Simon Newstead
    Pages 61-72 Open Access
  6. Juan Sanchez-Weatherby, Isabel Moraes
    Pages 73-89 Open Access
  7. Anna J. Warren, Danny Axford, Neil G. Paterson, Robin L. Owen
    Pages 105-117 Open Access
  8. Pierre Aller, Tian Geng, Gwyndaf Evans, James Foadi
    Pages 119-135 Open Access
  9. Kathrin Jaeger, Florian Dworkowski, Przemyslaw Nogly, Christopher Milne, Meitian Wang, Joerg Standfuss
    Pages 137-149
  10. Lan Zhu, Uwe Weierstall, Vadim Cherezov, Wei Liu
    Pages 151-160
  11. Philip C. Biggin, Matteo Aldeghi, Michael J. Bodkin, Alexander Heifetz
    Pages 161-181
  12. Pierre Aller, Tian Geng, Gwyndaf Evans, James Foadi
    Pages E3-E3
  13. Back Matter
    Pages 183-184

About this book


This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects.  Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins.

This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes:

  • Expression screening using fluorescent proteins
  • The use of detergents in membrane protein research
  • The use of NMR
  • Synchrotron developments in membrane protein structural biology
  • Visualisation and X-ray data collection of microcrystals
  • X-ray diffraction data analysis from multiple crystals
  • Serial millisecond crystallography
  • Serial femtosecond crystallography
  • Membrane protein structures in drug discovery

The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work.


Crystallization XFEL Molecular dynamics Human genome Electron Lasers

Editors and affiliations

  • Isabel Moraes
    • 1
  1. 1.Membrane Protein LaboratoryDiamond Light Source/Imperial College LondonDidcotUK

Bibliographic information

  • Book Title The Next Generation in Membrane Protein Structure Determination
  • Editors Isabel Moraes
  • Series Title Advances in Experimental Medicine and Biology
  • DOI
  • Copyright Information Springer International Publishing Switzerland 2016
  • Publisher Name Springer, Cham
  • eBook Packages Biomedical and Life Sciences Biomedical and Life Sciences (R0)
  • Hardcover ISBN 978-3-319-35070-7
  • Softcover ISBN 978-3-319-81715-6
  • eBook ISBN 978-3-319-35072-1
  • Series ISSN 0065-2598
  • Series E-ISSN 2214-8019
  • Edition Number 1
  • Number of Pages X, 184
  • Number of Illustrations 73 b/w illustrations, 0 illustrations in colour
  • Topics Protein Science
    Protein Structure
  • Buy this book on publisher's site
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