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© 2016

Advanced Technologies for Protein Complex Production and Characterization

  • M. Cristina Vega
  • Provides in-depth coverage of expression systems tailored for the production of multisubunit complexes

  • Gives extensive practical advice on how to design and conduct protein complex production experiments

  • Offers insightful discussions on characterization of purified protein complexes

Book

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 896)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Prokaryotic Expression Hosts

    1. Front Matter
      Pages 25-25
    2. Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald et al.
      Pages 27-42
    3. Harm Jan (Arjan) Snijder, Jonna Hakulinen
      Pages 59-77
    4. Mathieu Botte, Aurélien Deniaud, Christiane Schaffitzel
      Pages 79-95
    5. Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, M. Cristina Vega
      Pages 115-133
  4. Lower Eukaryotic Expression Hosts

    1. Front Matter
      Pages 135-135
    2. Francisco J. Fernández, Miguel López-Estepa, Javier Querol-García, M. Cristina Vega
      Pages 137-153
    3. Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez, M. Cristina Vega
      Pages 167-184
  5. Higher Eukaryotic Expression Hosts

    1. Front Matter
      Pages 185-185
    2. Thomas A. Kost, Christopher W. Kemp
      Pages 187-197
    3. Duygu Sari, Kapil Gupta, Deepak Balaji Thimiri Govinda Raj, Alice Aubert, Petra Drncová, Frederic Garzoni et al.
      Pages 199-215
    4. Michael R. Dyson
      Pages 217-224
    5. Bahar Baser, Joop van den Heuvel
      Pages 225-238

About this book

Introduction

This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists.

Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.

This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.

Keywords

Acid complexes Protein complexes Expression systems Eukaryotic expression systems Structural and biophysical characterization

Editors and affiliations

  • M. Cristina Vega
    • 1
  1. 1.Center for Biological ResearchSpanishNationalResearchCouncil(CIB-CSIC)MadridSpain

About the editors

Dr. M. Cristina Vega is a group leader at the Chemical and Physical Biology Department of the Center for Biological Research (CIB-CSIC) in Madrid, Spain. With substantial experience in macromolecular X-ray crystallography of protein complexes with biomedical relevance, she designs eukaryotic, yeast-based expression platforms tailored to protein complexes with a focus on cost-efficiency and improved performance. 

Bibliographic information

  • Book Title Advanced Technologies for Protein Complex Production and Characterization
  • Editors M. Cristina Vega
  • Series Title Advances in Experimental Medicine and Biology
  • Series Abbreviated Title Adv Exp Med Biol
  • DOI https://doi.org/10.1007/978-3-319-27216-0
  • Copyright Information Springer International Publishing Switzerland 2016
  • Publisher Name Springer, Cham
  • eBook Packages Biomedical and Life Sciences Biomedical and Life Sciences (R0)
  • Hardcover ISBN 978-3-319-27214-6
  • Softcover ISBN 978-3-319-80094-3
  • eBook ISBN 978-3-319-27216-0
  • Series ISSN 0065-2598
  • Series E-ISSN 2214-8019
  • Edition Number 1
  • Number of Pages XIII, 382
  • Number of Illustrations 16 b/w illustrations, 71 illustrations in colour
  • Topics Protein Science
    Protein Structure
    Systems Biology
  • Buy this book on publisher's site
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