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Advanced Technologies for Protein Complex Production and Characterization

  • M. Cristina Vega

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 896)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Prokaryotic Expression Hosts

    1. Front Matter
      Pages 25-25
    2. Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald et al.
      Pages 27-42
    3. Harm Jan (Arjan) Snijder, Jonna Hakulinen
      Pages 59-77
    4. Mathieu Botte, Aurélien Deniaud, Christiane Schaffitzel
      Pages 79-95
    5. Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, M. Cristina Vega
      Pages 115-133
  4. Lower Eukaryotic Expression Hosts

    1. Front Matter
      Pages 135-135
    2. Francisco J. Fernández, Miguel López-Estepa, Javier Querol-García, M. Cristina Vega
      Pages 137-153
    3. Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez, M. Cristina Vega
      Pages 167-184
  5. Higher Eukaryotic Expression Hosts

    1. Front Matter
      Pages 185-185
    2. Thomas A. Kost, Christopher W. Kemp
      Pages 187-197
    3. Duygu Sari, Kapil Gupta, Deepak Balaji Thimiri Govinda Raj, Alice Aubert, Petra Drncová, Frederic Garzoni et al.
      Pages 199-215
    4. Michael R. Dyson
      Pages 217-224
    5. Bahar Baser, Joop van den Heuvel
      Pages 225-238
  6. Plant Expression

    1. Front Matter
      Pages 239-239
    2. Franziska Hempel, Uwe G. Maier
      Pages 241-262
    3. Albert Ferrer, Monserrat Arró, David Manzano, Teresa Altabella
      Pages 263-285
  7. Complex Reconstitution

    1. Front Matter
      Pages 303-303
    2. Jérôme Basquin, Michael Taschner, Esben Lorentzen
      Pages 305-314
  8. Biophysical Methods to Assess Protein Complexes

  9. Back Matter
    Pages 369-382

About this book

Introduction

This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists.

Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.

This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.

Keywords

Acid complexes Protein complexes Expression systems Eukaryotic expression systems Structural and biophysical characterization

Editors and affiliations

  • M. Cristina Vega
    • 1
  1. 1.Center for Biological ResearchSpanishNationalResearchCouncil(CIB-CSIC)MadridSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-319-27216-0
  • Copyright Information Springer International Publishing Switzerland 2016
  • Publisher Name Springer, Cham
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-3-319-27214-6
  • Online ISBN 978-3-319-27216-0
  • Series Print ISSN 0065-2598
  • Series Online ISSN 2214-8019
  • Buy this book on publisher's site
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