Applications of Mass Spectrometry in Microbiology

From Strain Characterization to Rapid Screening for Antibiotic Resistance

  • Plamen Demirev
  • Todd R. Sandrin

Table of contents

  1. Front Matter
    Pages i-viii
  2. Plamen Demirev, Todd R. Sandrin
    Pages 1-10
  3. Methodology and Techniques

  4. Subspecies Discrimination

    1. Front Matter
      Pages 183-183
    2. Peter Lasch, Daniela Jacob, Silke R. Klee, Guido Werner
      Pages 203-234
    3. Melinda A. McFarland, Denis Andrzejewski, John H. Callahan
      Pages 257-271
  5. Drug Resistance Monitoring and Assays

    1. Front Matter
      Pages 273-273
    2. David Drissner, René Brunisholz, Ralph Schlapbach, Maria-Theresia Gekenidis
      Pages 275-303
    3. Jaroslav Hrabak, Vladimír Havlicek, Costas C. Papagiannitsis
      Pages 305-316
  6. Back Matter
    Pages 327-336

About this book


In the last quarter century, advances in mass spectrometry (MS) have been at the forefront of efforts to map complex biological systems including the human metabolome, proteome, and microbiome. All of these developments have allowed MS to become a well-established molecular level technology for microbial characterization. MS has demonstrated its considerable advantage as a rapid, accurate, and cost-effective method for microbial identification, compared to conventional phenotypic techniques. In the last several years, applications of MS for microbial characterization in research, clinical microbiology, counter-bioterrorism, food safety, and environmental monitoring have been documented in thousands of publications. Regulatory bodies in Europe, the US, and elsewhere have approved MS-based assays for infectious disease diagnostics. As of mid-2015, more than 3300 commercial MS systems for microbial identification have been deployed worldwide in hospitals and clinical labs.

While previous work has covered broader approaches in using MS to characterize microorganisms at the species level or above, this book focuses on strain-level and subtyping applications. In thirteen individual chapters, innovators, leaders and practitioners in the field from around the world have contributed to a comprehensive overview of current and next-generation approaches for MS-based microbial characterization at the subspecies and strain levels. Chapters include up-to-date reference lists as well as web-links to databases, recommended software, and other useful tools.

The emergence of new, antibiotic-resistant strains of human or animal pathogens is of extraordinary concern not only to the scientific and medical communities, but to the general public as well. Developments of novel MS-based assays for rapid identification of strains of antibiotic-resistant microorganisms are reviewed in the book as well. Microbiologists, bioanalytical scientists, infectious disease specialists, clinical laboratory and public health practitioners as well as researchers in universities, hospitals, government labs, and the pharmaceutical and biotechnology industries will find this book to be a timely and valuable resource.


Mass Spectrometry Strain-level focus Microorganism Characterization MS-based Assay infectious diseases

Editors and affiliations

  • Plamen Demirev
    • 1
  • Todd R. Sandrin
    • 2
  1. 1.Johns Hopkins UniversityApplied Physics LaboratoryLaurelUSA
  2. 2.Arizona State UniversitySchool of Math and Natural SciencesPhoenixUSA

Bibliographic information

Industry Sectors
Chemical Manufacturing