© 2015

Membrane Potential Imaging in the Nervous System and Heart

  • Marco Canepari
  • Dejan Zecevic
  • Olivier Bernus


  • Fully-expanded and updated Second Edition reflects all recent advances and new developments in the field

  • Comprehensively examines applications of membrane potential imaging, from the neuronal level to larger networks in the CNS and heart

  • Carefully curated images bring the content to life, tangibly demonstrating the abilities and applications of the technology


Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 859)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Principles of Membrane Potential-Imaging

    1. Front Matter
      Pages 1-1
    2. Oliver Braubach, Lawrence B. Cohen, Yunsook Choi
      Pages 3-26
  3. Membrane Potential Signals with Single Cell Resolution

    1. Front Matter
      Pages 55-55
    2. Marko Popovic, Kaspar Vogt, Knut Holthoff, Arthur Konnerth, Brian M. Salzberg, Amiram Grinvald et al.
      Pages 57-101
    3. Nadia Jaafari, Kaspar E. Vogt, Peter Saggau, Loew M. Leslie, Dejan Zecevic, Marco Canepari
      Pages 103-125
    4. W. N. Frost, C. J. Brandon, A. M. Bruno, M. D. Humphries, C. Moore-Kochlacs, T. J. Sejnowski et al.
      Pages 127-145
  4. Monitoring Activity of Networks and Large Neuronal Populations

    1. Front Matter
      Pages 147-147
    2. Kevin L. Briggman, William B. Kristan, Jesús E. González, David Kleinfeld, Roger Y. Tsien
      Pages 149-169
    3. Jianmin Liang, Weifeng Xu, Xinling Geng, Jian-young Wu
      Pages 171-196
    4. Brandon J. Wright, Meyer B. Jackson
      Pages 197-211
    5. Amiram Grinvald, David Omer, Shmuel Naaman, Dahlia Sharon
      Pages 243-271
  5. Monitoring Membrane Potential in the Heart

    1. Front Matter
      Pages 297-297
    2. Arkady Pertsov, Richard D. Walton, Olivier Bernus
      Pages 299-311
    3. Sarah A. Park, Richard A. Gray
      Pages 313-342
    4. Bastiaan J. Boukens, Sarah R. Gutbrod, Igor R. Efimov
      Pages 343-365
    5. Martin J. Bishop, Gernot Plank
      Pages 367-404

About this book


This volume discusses membrane potential imaging in the nervous system and in the heart and modern optical recording technology. Additionally, it covers organic and genetically-encoded voltage-sensitive dyes; membrane potential imaging from individual neurons, brain slices, and brains in vivo; optical imaging of cardiac tissue and arrhythmias; bio-photonics modelling . This is an expanded and fully-updated second edition, reflecting all the recent advances in this field.  

Twenty chapters, all authored by leading names in the field, are cohesively structured into four sections. The opening section focuses on the history and principles of membrane potential imaging and lends context to the following sections, which examine applications in single neurons, networks, large neuronal populations, and the heart. Topics discussed include population membrane potential signals in development of the vertebrate nervous system, use of membrane potential imaging from dendrites and axons, and depth-resolved optical imaging of cardiac activation and repolarization. The final section discusses the potential – and limitations – for new developments in the field, including new technology such as non-linear optics, advanced microscope designs, and genetically encoded voltage sensors.

Membrane Potential Imaging in the Nervous System and Heart is ideal for neurologists, electrophysiologists, cardiologists, and those who are interested in the applications and the future of membrane potential imaging.  


Brain Heart Membrane potential Voltage-Imaging Voltage-sensitive dye

Editors and affiliations

  • Marco Canepari
    • 1
  • Dejan Zecevic
    • 2
  • Olivier Bernus
    • 3
  1. 1.Laboratoire Interdisciplinaire de PhysiqueGrenobleFrance
  2. 2.Department of Cellular and Molecular PhysiologyYale University School of MedicineNew HavenUSA
  3. 3.L’Institut de rythmologie et mode´lisation cardiaque LIRYC Universite´ de BordeauxBordeauxFrance

About the editors

Marco Canepari (b. Milan 1970) is first class INSERM researcher (CR1) working at the Laboratoire Interdisciplinaire de Physique in Grenoble. He graduated in physics at the University of Genoa and received his PhD in biophysics from the International School for Advanced Studies in Trieste. He worked at the National Institute for Medical Research in London, at Yale University and at the University of Basel. Marco is expert on several optical techniques applied to neurophysiology. Marco and Dejan collaborated for a number of years using voltage-imaging and calcium imaging approaches to study mechanisms underlying synaptic plasticity.

Olivier Bernus (b. Tournai 1976) is Professor and Scientific Director at the Electrophysiology and Heart Modeling Institute LIRYC at the University of Bordeaux, France. He graduated in Mathematical Physics at the University of Ghent, Belgium and obtained his PhD in Physics from the same university. He subsequently worked as a postdoctoral fellow at SUNY Upstate Medical University, USA, and as a tenure track research fellow at the University of Leeds, UK. Olivier is an expert in cardiac optical imaging and is currently working on the development of novel techniques for three-dimensional depth-resolved optical imaging of cardiac electrical activity.

Dejan Zecevic (b. Belgrade 1948) is Senior Research Scientist at the Department of Cellular and Molecular Physiology, Yale University School of Medicine. He received the PhD in Biophysics from The University of Belgrade, Serbia and was trained in the laboratory of Dr Lawrence Cohen who initiated the field of voltage-sensitive dye recording. Dejan is the pioneer of intracellular voltage-sensitive dye imaging technique, a unique and a cutting edge technology for monitoring the membrane potential signaling in axons, dendrites and dendritic spines of individual nerve cells.

Bibliographic information

  • Book Title Membrane Potential Imaging in the Nervous System and Heart
  • Editors Marco Canepari
    Dejan Zecevic
    Olivier Bernus
  • Series Title Advances in Experimental Medicine and Biology
  • Series Abbreviated Title Advs Exp. Medicine, Biology
  • DOI
  • Copyright Information Springer International Publishing Switzerland 2015
  • Publisher Name Springer, Cham
  • eBook Packages Biomedical and Life Sciences Biomedical and Life Sciences (R0)
  • Hardcover ISBN 978-3-319-17640-6
  • Softcover ISBN 978-3-319-37144-3
  • eBook ISBN 978-3-319-17641-3
  • Series ISSN 0065-2598
  • Series E-ISSN 2214-8019
  • Edition Number 1
  • Number of Pages XI, 516
  • Number of Illustrations 35 b/w illustrations, 122 illustrations in colour
  • Topics Human Physiology
    Imaging / Radiology
  • Buy this book on publisher's site
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