© 2015

Advanced Photon Counting

Applications, Methods, Instrumentation

  • Peter Kapusta
  • Michael Wahl
  • Rainer Erdmann

Part of the Springer Series on Fluorescence book series (SS FLUOR, volume 15)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Gerald S. Buller, Robert J. Collins
    Pages 43-69
  3. Thomas Schönau, Sina Riecke, Andreas Bülter, Kristian Lauritsen
    Pages 71-87
  4. Kunihiko Ishii, Takuhiro Otosu, Tahei Tahara
    Pages 111-128
  5. Anders Barth, Lena Voith von Voithenberg, Don C. Lamb
    Pages 129-157
  6. Kristin S. Grußmayer, Dirk-Peter Herten
    Pages 159-190
  7. Maria J. Ruedas-Rama, Jose M. Alvarez-Pez, Luis Crovetto, Jose M. Paredes, Angel Orte
    Pages 191-223
  8. Rafal Fudala, Ryan M. Rich, Joe Kimball, Ignacy Gryczynski, Sangram Raut, Julian Borejdo et al.
    Pages 225-239
  9. Narain Karedla, Daja Ruhlandt, Anna M. Chizhik, Jörg Enderlein, Alexey I. Chizhik
    Pages 265-281
  10. Giuseppe Vicidomini, Ivàn Coto Hernàndez, Alberto Diaspro, Silvia Galiani, Christian Eggeling
    Pages 283-301
  11. Andreas Ahlrichs, Benjamin Sprenger, Oliver Benson
    Pages 319-341
  12. Dirk Grosenick
    Pages 343-365
  13. Back Matter
    Pages 367-370

About this book


This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.


Antibunchng Coincidence correlation Diamond defect centers FCS FLIM FRET Fluorescence correlation spectroscopy Fluorescence lifetime Optical tomography Single molecule spectroscopy Single photon detectors TCSPC Time interval analysis Time-correlated single photon counting

Editors and affiliations

  • Peter Kapusta
    • 1
  • Michael Wahl
    • 2
  • Rainer Erdmann
    • 3
  1. 1.J. Heyrovsky Institute of Physical ChemistryAcademy of Sciences of the Czech RepublicPrague 8Czech Republic
  2. 2.PicoQuant GmbHBerlinGermany
  3. 3.PicoQuant GmbHBerlinGermany

About the editors

Bibliographic information

Industry Sectors
Chemical Manufacturing


“The book is quite specialised and will likely be mainly of interest to researchers developing new photon counting techniques, fluorescence-based or not. … In addition to the constructors of photon counting systems, it could also be useful to some as a reference for comparing results, which could include those working in the life sciences as well as physicists and chemists.” (David Birch, Analytical and Bioanalytical Chemistry, Vol. 408, 2016)