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NMR-Bioassay Guided Isolation of the Natural 20S Proteasome Inhibitors from Photorhabdus Luminescens

A Novel NMR-Tool for Natural Product Detection

  • Martin Lorenz┬áStein

Part of the Springer Theses book series (Springer Theses)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Martin Lorenz Stein
    Pages 1-22
  3. Martin Lorenz Stein
    Pages 23-24
  4. Martin Lorenz Stein
    Pages 25-40
  5. Martin Lorenz Stein
    Pages 41-65
  6. Martin Lorenz Stein
    Pages 67-71

About this book

Introduction

Martin Stein's thesis describes a novel methodology for natural product discovery. Due to its high degree of reproducibility, robustness and sensitivity, the technique can be utilized to detect even trace amounts of bioactive substances in heterogeneous matrices such as fermentation broths or crude organic extracts. This research is thus relevant for a large number of researchers working in natural product discovery. Applications of this novel NMR-based approach include suitable environmental triggers for the induction of biosynthetic machineries. The author demonstrates the extraordinary value of this approach by the successful isolation of two potent inhibitors of the pharmaceutically relevant proteasome core particle from the insect pathogen photorhabdus luminescens. This thesis has led to a number of publications in high-impact journals.

Keywords

Inhibitor NMR Assay Natural Product Proteasome Screening Syrbactin Virulence

Authors and affiliations

  • Martin Lorenz┬áStein
    • 1
  1. 1.Faculty of Chemistry, Department of BiochemistryTechnical University of MunichGarchingGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-319-07914-1
  • Copyright Information Springer International Publishing Switzerland 2014
  • Publisher Name Springer, Cham
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-3-319-07913-4
  • Online ISBN 978-3-319-07914-1
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
  • Buy this book on publisher's site
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