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Techniques in Prion Research

  • Sylvain Lehmann
  • Jacques Grassi

Part of the Methods and Tools in Biosciences and Medicine book series (MTBM)

Table of contents

  1. Front Matter
    Pages I-X
  2. Kerstin Elfrink, Detlev Riesner
    Pages 4-15
  3. Sylvian Lehmann, Jerome Solassol, Veronique Perrier
    Pages 72-81
  4. Olivier Andréoletti
    Pages 82-96
  5. Moira E. Bruce, Aileen Boyle, Irene McConnell
    Pages 132-146
  6. David M. Taylor
    Pages 147-163
  7. Louise Kirby, James Hope
    Pages 164-175
  8. Roberto Chiesa, Luana Fioriti, Fabrizio Tagliavini, Mario Salmona, Gianluigi Forloni
    Pages 176-197
  9. Joaquin Castilla, Paula Saá, Claudio Soto
    Pages 198-213
  10. Back Matter
    Pages 214-221

About this book

Introduction

Prion diseases, also known as Transmissible Spongiform Encephalopathies (TSEs), exist in both humans (Creutzfeldt-Jakob disease (CJD)) and animals (scrapie, bovine spongiform encephalopathy (BSE), chronic wasting disease) and have the unique property of being infectious, sporadic or genetic in origin.

Although the precise nature of the infectious agent responsible for TSEs is not definitely identified, it is now clearly demonstrated that a protein named PrP (for Prion Protein) plays a critical role in the transmission and pathogenesis of TSEs.

This book provides the general description as well as the details of the techniques currently used for the study of prion diseases. Taking into account the pivotal role played by PrP it is not surprising that many Chapters of this book deal with the purification, the detection and the characterization of the different forms of this protein. In addition, in vitro, cellular and animal models specifically adapted to the study of TSEs, as well as bio-safety procedures are described. Each Chapter is written by scientists involved for many years in their respective domain of prion biology who give the best of their knowledge in this technical document. This volume is a very useful tool for any laboratory which recently decided to contribute to the study of TSEs as well as for teams already engaged in this field for many years but interested in extending their technical capacity toward new methods.

Features:

Purification of PrPC and the Pathological Isoform of Prion Protein (PrPsc or PrP-res)

Animal Models of TSEs

Cell Culture Models of TSEs

PrPsc Immunohistochemistry

Western Immunoblotting Techniques

Antibody Production and ELISA

TSE Strain Typing in Mice

Biosafety and Decontamination Procedures

Cell-free Conversion of Prion Proteins

Cytotoxicity of PrP Peptides

Cyclic Amplification of Prion Protein Misfolding<

Of interest to:

Researchers and clinicians in the fields of cell biology, biomedicine, neuroscience/neuropathology, veterinary medicine and biochemistry.

Keywords

Animal Models Antibody Production BSE CJD Cell Culture Models Cytotoxicity Decontamination PrP Purification antibody antibody production biochemistry biology brain chemistry cytotoxicity diseases ELISA neuroscience pathogenesis prion diseases protein proteins Transmissible spongiform encephalopathies

Editors and affiliations

  • Sylvain Lehmann
    • 1
  • Jacques Grassi
    • 2
  1. 1.Institut de Génétique HumaineCNRS U.P.R. 1142Montpellier Cedex 5France
  2. 2.Service de Pharmacologie et d’ImmunologieBâtiment 136, CEA/SaclayGif sur Yvette CedexFrance

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