© 2020

Practical Immunocytochemistry in Diagnostic Cytology


  • Provides practical information on how to prepare cytologic samples to obtain reliable ICC stain results

  • Chapters include descriptions of the cytomorphology of the most common tumors followed by practical ICC staining patterns for each entity

  • Written by experts in the field


Table of contents

  1. Front Matter
    Pages i-xv
  2. Eugenio Leonardo, Ricardo H. Bardales
    Pages 1-5
  3. Eugenio Leonardo, Ricardo H. Bardales
    Pages 7-40
  4. Eugenio Leonardo, Ricardo H. Bardales
    Pages 41-67
  5. Eugenio Leonardo, Ricardo H. Bardales
    Pages 69-111
  6. Eugenio Leonardo, Ricardo H. Bardales
    Pages 113-152
  7. Eugenio Leonardo, Ricardo H. Bardales
    Pages 153-163
  8. Back Matter
    Pages 165-174

About this book


This book provides current information for a judicious use of conventional and molecular ICC markers performed in cytologic samples to help in the cost-effective diagnosis of superficial and deep-seated masses. By providing both an eminently practical information for appropriate technical preparation of cytologic samples and a systematic exposure of the immunophenotypic and biomolecular characteristics of normal and pathological cells, the reported morphological-molecular findings highlight the characteristics of different lesions that are useful for the practical solution of a particular differential diagnosis. Chapters include description of the cytomorphology followed by a practical ICC staining pattern for each entity. Cytologic materials include serous effusions, exfoliative cytology, fine needle aspiration cytology of superficial and deep-seated masses, and squash preparation from central nervous system masses. 

Practical Immunocytochemistry in Diagnostic Cytology serves as a valuable resource towards understanding critical differences between tests performed on tissue samples and those carried out on cytologic samples.


cytological samples Antigen retrieval hyperplastic mesothelium Respiratory Tract Biliary tract Mediastinum immunostains

Authors and affiliations

  1. 1.First level Pathologist Doctor, San Lazzaro HospitalAlbaItaly
  2. 2.Director Fine Needle Aspiration Service, Precision Pathology / Outpatient Pathology AssociatesSacramentoUSA

About the authors

Eugenio Leonardo
First level Pathologist Doctor
San Lazzaro Hospital
Alba, Italy

Ricardo H. Bardales
Director Fine Needle Aspiration Service
Precision Pathology / Outpatient Pathology Associates
Sacramento, CA

Bibliographic information

  • Book Title Practical Immunocytochemistry in Diagnostic Cytology
  • Authors Eugenio Leonardo
    Ricardo H. Bardales
  • DOI
  • Copyright Information Springer Nature Switzerland AG 2020
  • Publisher Name Springer, Cham
  • eBook Packages Medicine Medicine (R0)
  • Hardcover ISBN 978-3-030-46655-8
  • Softcover ISBN 978-3-030-46658-9
  • eBook ISBN 978-3-030-46656-5
  • Edition Number 1
  • Number of Pages XV, 174
  • Number of Illustrations 0 b/w illustrations, 484 illustrations in colour
  • Topics Pathology
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals