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Table of contents

  1. Front Matter
    Pages i-xi
  2. Luís Manuel Couto Oliveira, Valery Victorovich Tuchin
    Pages 1-15
  3. Luís Manuel Couto Oliveira, Valery Victorovich Tuchin
    Pages 17-34
  4. Luís Manuel Couto Oliveira, Valery Victorovich Tuchin
    Pages 35-48
  5. Luís Manuel Couto Oliveira, Valery Victorovich Tuchin
    Pages 49-59
  6. Luís Manuel Couto Oliveira, Valery Victorovich Tuchin
    Pages 61-77
  7. Luís Manuel Couto Oliveira, Valery Victorovich Tuchin
    Pages 79-105
  8. Luís Manuel Couto Oliveira, Valery Victorovich Tuchin
    Pages 107-138
  9. Luís Manuel Couto Oliveira, Valery Victorovich Tuchin
    Pages 139-161
  10. Luís Manuel Couto Oliveira, Valery Victorovich Tuchin
    Pages 163-172
  11. Back Matter
    Pages 173-177

About this book

Introduction

This book describes the Optical Immersion Clearing method and its application to acquire information with importance for clinical practice and various fields of biomedical engineering. The method has proved to be a reliable means of increasing tissue transparency, allowing the investigator or surgeon to reach deeper tissue layers for improved imaging and laser surgery. This result is obtained by partial replacement of tissue water with an active optical clearing agent (OCA) that has a higher refractive index and is a better match for the refractive index of other tissue components. Natural tissue scattering is thereby reduced. An exponential increase in research using this method has occurred in recent years, and new applications have emerged, both in clinical practice and in some areas of biomedical engineering. Recent research has revealed that treating ex vivo tissues with solutions containing active OCAs in different concentrations produces experimental data to characterize drug delivery or to discriminate between normal and pathological tissues. The obtained drug diffusion properties are of interest for the pharmaceutical and organ preservation industry. Similar data can be estimated with particular interest for food preservation. The free water content evaluation is also of great interest since it facilitates the characterization of tissues to discriminate pathologies. An interesting new application that is presented in the book regards the creation of two optical windows in the ultraviolet spectral range through the application of the immersion method. These induced transparency windows open the possibility to diagnose and treat pathologies with ultraviolet light. This book presents photographs from the tissues we have studied and figures that represent the experimental setups used. Graphs and tables are also included to show the numerical results obtained in the sequential calculations performed.

Keywords

Optical Immersion Clearing method Optical Clearing Agents Optical Clearing mechanisms Measurements during Optical Clearing increasing tissue transparency Diffusion properties of chemicals in biological tissues free water content in biological tissues Pathology diagnosis with optical methods optical properties of biological tissues

Authors and affiliations

  • Luís Manuel Couto Oliveira
    • 1
  • Valery Victorovich Tuchin
    • 2
  1. 1.Physics Department and Center for Innovation in Engineering and Industrial TechnologyPolytechnic Institute of Porto – School of EngineeringPortoPortugal
  2. 2.Department of Optics and Biophotonics, Bach Institute of BiochemistrySaratov State University, Institute of Precision Mechanics and Control of the RAS, Research Center of Biotechnology of the RAS, Moscow, Tomsk State University, Tomsk & ITMO University, St. PetersburgSaratovRussia

Bibliographic information

Industry Sectors
Pharma