© 2019

Label-Free Super-Resolution Microscopy

  • Vasily Astratov

Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Chenfei Hu, Gabriel Popescu
    Pages 1-24
  3. Richard W. Taylor, Vahid Sandoghdar
    Pages 25-65
  4. Nancy Rahbany, Ignacio Izeddin, Valentina Krachmalnicoff, Rémi Carminati, Gilles Tessier, Yannick De Wilde
    Pages 113-136
  5. Paolo Bianchini, Giulia Zanini, Alberto Diaspro
    Pages 171-193
  6. Katsumasa Fujita
    Pages 195-211
  7. Hadar Pinhas, Yossef Danan, Amihai Meiri, Omer Wagner, Asaf Shahmoon, Tali Ilovitsh et al.
    Pages 213-237
  8. Tushar C. Jagadale, Shi-Wei Chu
    Pages 239-259
  9. Omer Tzang, Dror Hershkovitz, Ori Cheshnovsky
    Pages 261-287
  10. Igor I. Smolyaninov, Vera N. Smolyaninova
    Pages 313-343
  11. Emroz Khan, Evgenii Narimanov
    Pages 345-369
  12. Zengbo Wang, Boris Luk’yanchuk
    Pages 371-406
  13. Alexey V. Maslov, Vasily N. Astratov
    Pages 407-441
  14. Stephane Perrin, Sylvain Lecler, Paul Montgomery
    Pages 443-469
  15. Back Matter
    Pages 471-487

About this book


This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.


Label-free Imaging Optical Nanoscopy Label-free Super-resolution Label-free Nanoscopy Super-resolved Fluorescence Microscopy Label-free Super-resolution Microscopy Superresolution Microscopy Microspherical Nanoscopy Plasmonics in Super-Resolution Imaging Interferometric Scattering Microscopy

Editors and affiliations

  • Vasily Astratov
    • 1
  1. 1.University of North Carolina at CharlotteCharlotteUSA

About the editors

Vasily N. Astratov has been professor of Physics and Optical Science at the University of North Carolina-Charlotte since 2002. In 1986, he received his Ph.D. degree from the A.F. Ioffe Physical-Technical Institute in Russia. Since joining UNC-Charlotte in 2002, his research has been devoted to a new field of study which he has named "microspherical photonics" to describe the applications of dielectric microspheres in super-resolution microscopy, resonant light forces, photonic nanojets, and photonic molecules. In his lab, he proposed and developed the methods of super-resolution imaging based on using high-index dielectric microspheres immersed in liquids or in elastomeric slabs. His methods are widely used by many groups worldwide for imaging subcellular structures, viruses, and nanoplasmonic structures. He also observed giant light forces exerted on microspheres under resonant conditions with their whispering gallery modes. This observation builds upon earlier pioneering work of Arthur Ashkin and Joseph M. Dziedzic on optical forces exerted on microdroplets. Previously, in the mid-1990s he pioneered studies of synthetic opals as novel three-dimensional photonic crystals for visible light. He has authored and co-authored about 180 research publications and 15 patents which have been cited more than 6000 times.

Bibliographic information


“A thorough and cutting-edge reference of the modern advances in microscopy, the book is valuable reading for researchers and physicists.” (Silvano Donati, Optics & Photonics News, March 19, 2020)