© 2019

Activity-Based Protein Profiling

  • Benjamin F. Cravatt
  • Ku-Lung Hsu
  • Eranthie Weerapana


  • Provides new insights into activity-based protein profiling (ABPP), which uses chemical probes to profile the functional state of enzymes in native biological systems

  • Focuses on the application of ABPP methodologies to processes relevant to microbiology and immunology

  • Helps readers identify the posttranslational regulatory mechanisms that modulate protein function


Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 420)

Table of contents

  1. Front Matter
    Pages i-x
  2. Shabnam Sharifzadeh, Joshua D. Shirley, Erin E. Carlson
    Pages 23-48
  3. Hiren V. Patel, Michael Li, Jessica C. Seeliger
    Pages 49-72
  4. Yekaterina Kovalyova, Stavroula K. Hatzios
    Pages 73-91
  5. Gerbrand J. van der Heden van Noort, Huib Ovaa
    Pages 111-130
  6. Geneviève F. Desrochers, John Paul Pezacki
    Pages 131-154
  7. Henry J. Benns, Edward W. Tate, Matthew A. Child
    Pages 155-174
  8. Adam L. Borne, Tao Huang, Rebecca L. McCloud, Boobalan Pachaiyappan, Timothy N. J. Bullock, Ku-Lung Hsu
    Pages 175-210
  9. Venkatesh V. Nemmara, Paul R. Thompson
    Pages 233-251
  10. Suravi Chakrabarty, Jan Pascal Kahler, Merel A. T. van de Plassche, Roeland Vanhoutte, Steven H. L. Verhelst
    Pages 253-281
  11. Sebastiaan T. A. Koenders, Berend Gagestein, Mario van der Stelt
    Pages 283-319
  12. Fumihiro Ishikawa, Genzoh Tanabe, Hideaki Kakeya
    Pages 321-349
  13. Daniel K. Nomura, Thomas J. Maimone
    Pages 351-374
  14. Keriann M. Backus
    Pages 375-417

About this book


This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.


Penicillin-binding proteins Poly-ADP-ribosylation Host-virus interactions Intramembrane proteases Cysteine profiling Lysine profiling Endocannabinoid enzymes ABPP

Editors and affiliations

  • Benjamin F. Cravatt
    • 1
  • Ku-Lung Hsu
    • 2
  • Eranthie Weerapana
    • 3
  1. 1.Department of ChemistryThe Scripps Research InstituteLa JollaUSA
  2. 2.Department of ChemistryUniversity of VirginiaCharlottesvilleUSA
  3. 3.Department of ChemistryBoston CollegeChestnut HillUSA

About the editors

Benjamin F. Cravatt, Ph.D.

Department of Chemistry

The Scripps Research Institute

La Jolla, CA, USA


Ku-Lung Hsu Ph.D.

University of Virginia School of Medicine

Department of Chemistry

Charlottesville, VA, USA


Eranthie Weerapana Ph.D.

Boston College

Merkert Chemistry

Chestnut Hill, MA, USA

Bibliographic information

  • Book Title Activity-Based Protein Profiling
  • Editors Benjamin F. Cravatt
    Ku-Lung Hsu
    Eranthie Weerapana
  • Series Title Current Topics in Microbiology and Immunology
  • Series Abbreviated Title Current Topics in Microbiology and Immunology
  • DOI
  • Copyright Information Springer Nature Switzerland AG 2019
  • Publisher Name Springer, Cham
  • eBook Packages Biomedical and Life Sciences Biomedical and Life Sciences (R0)
  • Hardcover ISBN 978-3-030-11142-7
  • eBook ISBN 978-3-030-11143-4
  • Series ISSN 0070-217X
  • Series E-ISSN 2196-9965
  • Edition Number 1
  • Number of Pages X, 417
  • Number of Illustrations 45 b/w illustrations, 70 illustrations in colour
  • Topics Immunology
    Medical Microbiology
  • Buy this book on publisher's site
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