Chloroplast Biotechnology

Methods and Protocols

  • Pal Maliga

Part of the Methods in Molecular Biology book series (MIMB, volume 1132)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Background

    1. Front Matter
      Pages 1-1
    2. Tracey A. Ruhlman, Robert K. Jansen
      Pages 3-38
    3. Robert J. Henry, Nicole Rice, Mark Edwards, Catherine J. Nock
      Pages 39-46
    4. Jennifer Ortelt, Gerhard Link
      Pages 47-72
    5. Masahiro Sugiura
      Pages 73-91
    6. Elisabeth A. Mudd, Panagiotis Madesis, Elena Martin Avila, Anil Day
      Pages 107-123
  3. Nicotiana tabacum the Model Species of Chloroplast Biotechnology

    1. Front Matter
      Pages 145-145
    2. Areli Herrera Díaz, Hans-Ulrich Koop
      Pages 165-175
    3. Pierluigi Barone, Xing-Hai Zhang, Jack M. Widholm
      Pages 187-203
    4. Sithichoke Tangphatsornruang, John C. Gray
      Pages 221-234
  4. Crop Specific Plastid Transformation Protocols

    1. Front Matter
      Pages 263-263
    2. Stephanie Ruf, Ralph Bock
      Pages 265-276
    3. Elena Martin Avila, Anil Day
      Pages 277-293
    4. Vladimir T. Valkov, Daniela Gargano, Nunzia Scotti, Teodoro Cardi
      Pages 295-303
    5. Kailash C. Bansal, Ajay K. Singh
      Pages 305-316
    6. Cilia L. C. Lelivelt, Kees M. P. van Dun, C. Bastiaan de Snoo, Matthew S. McCabe, Bridget V. Hogg, Jacqueline M. Nugent
      Pages 317-330
    7. Manuel Dubald, Ghislaine Tissot, Bernard Pelissier
      Pages 345-354
    8. Menq-Jiau Tseng, Ming-Te Yang, Wan-Ru Chu, Cheng-Wei Liu
      Pages 355-366
    9. Francesca De Marchis, Michele Bellucci
      Pages 367-373
    10. Shaochen Xing, Zhengyi Wei, Yunpeng Wang, Yanzhi Liu, Chunjing Lin
      Pages 375-387
  5. Plastid Transformation in Algae

    1. Front Matter
      Pages 389-389
    2. Daniel Barrera, Javier Gimpel, Stephen Mayfield
      Pages 391-399
    3. Chloe Economou, Thanyanan Wannathong, Joanna Szaub, Saul Purton
      Pages 401-411
    4. Jean-David Rochaix, Raymond Surzycki, Silvia Ramundo
      Pages 413-424
  6. Plastid Transformation in Bryophyte

    1. Front Matter
      Pages 425-425
  7. Back Matter
    Pages 449-452

About this book


In Chloroplast Biotechnology: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology.  Chapters focus on essential background information, applications in tobacco, and protocols for plastid transformation in crops, and Chlamydomonas and Bryophytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls.


Authoritative and practical,  Chloroplast Biotechnology: Methods and Protocols seeks to aid scientists who study chloroplast molecular biology as well as those interested in applications in agriculture, industrial biotechnology, and healthcare.


Plastid functions amino acid lipid photosynthesis pigment biosynthesis plastid biology plastid genome plastid transformation in Bryophytes plastid transformation in Chlamydomonas plastid transformation in crops starch tissue culture tobacco

Editors and affiliations

  • Pal Maliga
    • 1
  1. 1.Rutgers UniversityWaksman Inst.of MicrobiologyPiscatawayUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-994-9
  • Online ISBN 978-1-62703-995-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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