Monoclonal Antibodies

Methods and Protocols

  • Vincent Ossipow
  • Nicolas Fischer

Part of the Methods in Molecular Biology book series (MIMB, volume 1131)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Antibody Generation

    1. Front Matter
      Pages 1-1
    2. Giovanni Magistrelli, Pauline Malinge
      Pages 3-20
    3. Hyung-Yong Kim, Alexander Stojadinovic, Mina J. Izadjoo
      Pages 33-45
    4. Efthalia Chronopoulou, Alejandro Uribe-Benninghoff, Cindi R. Corbett, Jody D. Berry
      Pages 47-70
    5. Pi-Chen Yam, Katherine L. Knight
      Pages 71-79
    6. Alejandro Uribe-Benninghoff, Teresa Cabral, Efthalia Chronopoulou, Jody D. Berry, Cindi R. Corbett
      Pages 81-103
    7. Patrick Koenig, Germaine Fuh
      Pages 133-149
    8. Joyce Hui-Yuen, Siva Koganti, Sumita Bhaduri-McIntosh
      Pages 183-189
  3. Antibody Expression and Purification

    1. Front Matter
      Pages 205-205
    2. Nicole Müller-Sienerth, Cécile Crosnier, Gavin J. Wright, Nicole Staudt
      Pages 229-240
    3. Larry J. Cummings, Russell G. Frost, Mark A. Snyder
      Pages 241-251
    4. Pauline Malinge, Giovanni Magistrelli
      Pages 253-261
    5. Krista Alvin, Jianxin Ye
      Pages 263-271
    6. Martin Siegemund, Fabian Richter, Oliver Seifert, Felix Unverdorben, Roland E. Kontermann
      Pages 273-295
    7. Emil Bujak, Mattia Matasci, Dario Neri, Sarah Wulhfard
      Pages 315-334
  4. Anitbody Characterization and Modification

    1. Front Matter
      Pages 335-335
    2. Eltaf Alamyar, Véronique Giudicelli, Patrice Duroux, Marie-Paule Lefranc
      Pages 337-381
    3. Robyn L. Stanfield
      Pages 395-406
    4. Hyung-Yong Kim, Alexander Stojadinovic, Mina J. Izadjoo
      Pages 407-420
    5. Terumi Midoro-Horiuti, Randall M. Goldblum
      Pages 421-426
    6. Nicola Clementi, Nicasio Mancini, Elena Criscuolo, Francesca Cappelletti, Massimo Clementi, Roberto Burioni
      Pages 427-446
    7. Terumi Midoro-Horiuti, Randall M. Goldblum
      Pages 477-484
    8. Anna-Luisa Volk, Francis Jingxin Hu, Johan Rockberg
      Pages 485-500
    9. Marie Corbier, Delphine Schrag, Sylvain Raimondi
      Pages 501-506
    10. Delphine Schrag, Marie Corbier, Sylvain Raimondi
      Pages 507-512
    11. Stefanie Derer, Christian Kellner, Thies Rösner, Katja Klausz, Pia Glorius, Thomas Valerius et al.
      Pages 525-540
  5. Applications of Monoclonal Antibodies

    1. Front Matter
      Pages 541-541
    2. Carl A. K. Borrebaeck, Christer Wingren
      Pages 563-571
  6. Back Matter
    Pages 573-575

About this book


Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.


aggregated monoclonals antigen generation crystallization chaperoning downstream purification of IgG immunofluorescence monoclonal antibodies monoclonal antibody characterization monoclonal antibody expression monoclonal antibody generation monoclonal antibody modification monoclonal antibody purification mutagenesis phage- or bacterial display solid-state arrays unstable monoclonals

Editors and affiliations

  • Vincent Ossipow
    • 1
  • Nicolas Fischer
    • 2
  1. 1.Département de BiochimieUniversity of GenevaGenevaSwitzerland
  2. 2.NovImmune SAPlan-les-OuatesSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-991-8
  • Online ISBN 978-1-62703-992-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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