Spliceosomal Pre-mRNA Splicing

Methods and Protocols

  • Klemens J. Hertel

Part of the Methods in Molecular Biology book series (MIMB, volume 1126)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introductory Chapters

    1. Front Matter
      Pages 1-1
    2. Somsakul Pop Wongpalee, Shalini Sharma
      Pages 3-12
    3. Scott William Roy, Manuel Irimia
      Pages 13-33
    4. Ni-ting Chiou, Kristen W. Lynch
      Pages 35-43
    5. Stacey D. Wagner, J. Andrew Berglund
      Pages 45-54
    6. Miguel B. Coelho, Christopher W. J. Smith
      Pages 55-82
    7. Evan C. Merkhofer, Peter Hu, Tracy L. Johnson
      Pages 83-96
    8. Nazmul Haque, Shalini Oberdoerffer
      Pages 97-113
  3. Methods Chapters

    1. Front Matter
      Pages 115-115
    2. Chiu-Ho T. Webb, Klemens J. Hertel
      Pages 117-121
    3. Elizabeth A. Dunn, Stephen D. Rader
      Pages 123-135
    4. Martha R. Stark, Stephen D. Rader
      Pages 137-149
    5. Maliheh Movassat, William F. Mueller, Klemens J. Hertel
      Pages 151-160
    6. William F. Mueller, Klemens J. Hertel
      Pages 161-168
    7. Janine O. Ilagan, Melissa S. Jurica
      Pages 179-192
    8. Eric Allemand, Michelle L. Hastings
      Pages 205-216
    9. Eric G. Anderson, Aaron A. Hoskins
      Pages 217-241
    10. Sarah A. Smith, Kristen W. Lynch
      Pages 243-255
    11. Noa Neufeld, Yehuda Brody, Yaron Shav-Tal
      Pages 257-269
    12. Joonbae Seo, Eric W. Ottesen, Ravindra N. Singh
      Pages 271-283
    13. Munshi Azad Hossain, Tracy L. Johnson
      Pages 285-298
    14. Ryan Percifield, Daniel Murphy, Peter Stoilov
      Pages 299-313
    15. Nicole I. Bieberstein, Korinna Straube, Karla M. Neugebauer
      Pages 315-323
    16. Rodger B. Voelker, William A. Cresko, J. Andrew Berglund
      Pages 325-340
    17. Gael P. Alamancos, Eneritz Agirre, Eduardo Eyras
      Pages 357-397
    18. Chengguo Yao, Lingjie Weng, Yongsheng Shi
      Pages 399-410
    19. Yoseph Barash, Jorge Vaquero Garcia
      Pages 411-423
  4. Back Matter
    Pages 425-427

About this book


Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo, manipulation of splicing events, and high-throughput and bioinformatic analyses of alternative splicing.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.


Alternative splicing Functional mRNAs Multiple protein isoforms Nuclear pre-mRNAs Protein coding sequences

Editors and affiliations

  • Klemens J. Hertel
    • 1
  1. 1.University of CaliforniaIrvineUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-979-6
  • Online ISBN 978-1-62703-980-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals