Methods and Protocols

  • Joanna Rorbach
  • Agnieszka J. Bobrowicz

Part of the Methods in Molecular Biology book series (MIMB, volume 1125)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. 3′-End Processing in Eukaryotes

    1. Front Matter
      Pages 1-1
    2. Thomas J. Sweet, Donny D. Licatalosi
      Pages 3-12
    3. Deepak P. Patil, Baskar Bakthavachalu, Daniel R. Schoenberg
      Pages 13-23
    4. Man Chun Lee, Amrei Jänicke, Traude Helene Beilharz
      Pages 25-42
    5. François M. Sement, Dominique Gagliardi
      Pages 43-51
    6. Olga Coll, Ana Villalba, Fátima Gebauer
      Pages 53-63
    7. Takashi Nagaike, James L. Manley
      Pages 65-74
    8. Özgül Persil Çetinkol
      Pages 81-108
    9. Ioannis Kavakiotis, George Tzanis, Ioannis Vlahavas
      Pages 131-140
    10. Antonio Lembo, Paolo Provero
      Pages 141-144
    11. Ashley L. Cornett, Carol S. Lutz
      Pages 157-167
    12. Yu Zhou, Hai-Ri Li, Jie Huang, Ge Jin, Xiang-Dong Fu
      Pages 169-178
  3. Bacteria and Organelles

    1. Front Matter
      Pages 209-209
    2. Joanna Rorbach, Agnieszka Bobrowicz, Sarah Pearce, Michal Minczuk
      Pages 211-227
    3. Bijoy K. Mohanty, Sidney R. Kushner
      Pages 229-249
  4. Deadenylation and Decay

    1. Front Matter
      Pages 287-287
    2. Xiaokan Zhang, Frida E. Kleiman, Emral Devany
      Pages 289-296
    3. Mandy Jeske, Claudia Temme, Elmar Wahle
      Pages 297-311
    4. Claudia Temme, Elmar Wahle
      Pages 313-324
    5. Joel Hrit, Nathan Raynard, Jamie Van Etten, Kamya Sankar, Adam Petterson, Aaron C. Goldstrohm
      Pages 325-339
    6. Motoaki Wakiyama, Shigeyuki Yokoyama
      Pages 341-351
    7. Julie A. Dougherty, Roshan Mascarenhas, Daniel R. Schoenberg
      Pages 353-371
  5. Olga Coll, Ana Villalba, Fátima Gebauer
    Pages E1-E1
  6. Deepak P. Patil, Baskar Bakthavachalu, Daniel R. Schoenberg
    Pages E3-E3
  7. Back Matter
    Pages 373-377

About this book


In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation.  Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Deadenylation RNA stability RNA translation alternative polyadenylation eukaryotic polyadenylation mRNA mammalian gene expression polyadenylation polyadenylation sites

Editors and affiliations

  • Joanna Rorbach
    • 1
  • Agnieszka J. Bobrowicz
    • 2
  1. 1.MRC Mitochondrial Biology UnitMitochondrial Genetics GroupCambridgeUnited Kingdom
  2. 2.Mitochondrial Research GroupNewcastle University The Medical SchoolNewcastle upon TyneUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-970-3
  • Online ISBN 978-1-62703-971-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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