Neutrophil Methods and Protocols

  • Mark T. Quinn
  • Frank R. DeLeo

Part of the Methods in Molecular Biology book series (MIMB, volume 1124)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Neutrophils: An Overview

    1. Front Matter
      Pages 1-1
    2. Harry L. Malech, Frank R. DeLeo, Mark T. Quinn
      Pages 3-10
  3. Neutrophil Isolation and Subcellular Fractionation

    1. Front Matter
      Pages 11-11
    2. William M. Nauseef
      Pages 13-18
    3. Daniel W. Siemsen, Natalia Malachowa, Igor A. Schepetkin, Adeline R. Whitney, Liliya N. Kirpotina, Benfang Lei et al.
      Pages 19-37
    4. Karin Christenson, Lena Björkman, Lisa Davidsson, Anna Karlsson, Per Follin, Claes Dahlgren et al.
      Pages 39-52
    5. Stine Novrup Clemmensen, Lene Udby, Niels Borregaard
      Pages 53-76
  4. Biochemistry, Biology and Signal Transduction of Neutrophils

    1. Front Matter
      Pages 77-77
    2. Richard T. Jennings, Ulla G. Knaus
      Pages 79-88
    3. Susan Sergeant, Linda C. McPhail
      Pages 89-105
    4. Maurice B. Hallett, Maha Al-Jumaa, Sharon Dewitt
      Pages 107-120
    5. Deri Morgan, Thomas E. DeCoursey
      Pages 121-158
    6. David A. Dorward, Adriano G. Rossi, Ian Dransfield, Christopher D. Lucas
      Pages 159-180
    7. Iraj Laffafian, Kimberly J. Lewis, K. Benjamin Masterman, Maurice B. Hallett
      Pages 181-187
    8. Colin L. Sweeney, Randall K. Merling, Uimook Choi, Debra Long Priel, Douglas B. Kuhns, Hongmei Wang et al.
      Pages 189-206
  5. Neutrophil Adhesion and Chemotaxis

    1. Front Matter
      Pages 207-207
    2. Richard T. Jennings, Ulla G. Knaus
      Pages 209-218
    3. Pui-ying Lam, Robert S. Fischer, William D. Shin, Clare M. Waterman, Anna Huttenlocher
      Pages 219-233
    4. Stuart M. Altman, Neha Dixit, Scott I. Simon
      Pages 235-248
  6. Neutrophil Phagocytosis and Bactericidal Activity

    1. Front Matter
      Pages 249-249
    2. Benjamin E. Steinberg, Marco A. O. Magalhaes, Sergio Grinstein
      Pages 269-277
    3. Heather A. Parker, Nicholas J. Magon, Jessie N. Green, Mark B. Hampton, Christine C. Winterbourn
      Pages 291-306
    4. Alejandro Sanchez Gonzalez, Bart W. Bardoel, Christopher J. Harbort, Arturo Zychlinsky
      Pages 307-318
  7. NADPH Oxidase and Production of Reactive Oxygen Species

    1. Front Matter
      Pages 319-319
    2. Johan Bylund, Halla Björnsdottir, Martina Sundqvist, Anna Karlsson, Claes Dahlgren
      Pages 321-338
    3. Margarita Hurtado-Nedelec, Karama Makni-Maalej, Marie-Anne Gougerot-Pocidalo, Pham My-Chan Dang, Jamel El-Benna
      Pages 405-412
    4. Sahra Amel Belambri, Pham My-Chan Dang, Jamel El-Benna
      Pages 427-433
  8. Analysis of Neutrophil Gene Expression and Transcription Factors

    1. Front Matter
      Pages 435-435
    2. Scott D. Kobayashi, Daniel E. Sturdevant, Frank R. DeLeo
      Pages 437-450
    3. Nicola Tamassia, Marco A. Cassatella, Flavia Bazzoni
      Pages 451-467
    4. Patrick P. McDonald, Richard D. Ye
      Pages 485-498
  9. Neutrophil Defects and Diagnosis

    1. Front Matter
      Pages 499-499
    2. Mary C. Dinauer
      Pages 501-515
    3. Houda Zghal Elloumi, Steven M. Holland
      Pages 517-535
  10. Back Matter
    Pages 547-551

About this book


Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells, and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity, and extracellular trap formation; and protocols for investigating neutrophil adhesion, chemotaxis, and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.


NADPH oxidase bactericidal activity gene expression human neutrophil neutrophil neutrophil apoptosis neutrophil phagocytosis

Editors and affiliations

  • Mark T. Quinn
    • 1
  • Frank R. DeLeo
    • 2
  1. 1.Montana State University Dept. Veterinary Molecular BiologyBozemanUSA
  2. 2.Lab. Human Bacterial PathogenesisNational Institutes of Health Nat. Inst. Allergy & Infectious DiseasesHamiltonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-844-7
  • Online ISBN 978-1-62703-845-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals
Public Health
Internal Medicine & Dermatology