Ras Signaling

Methods and Protocols

  • Lorenza Trabalzini
  • Saverio Francesco Retta

Part of the Methods in Molecular Biology book series (MIMB, volume 1120)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Luca Goitre, Eliana Trapani, Lorenza Trabalzini, Saverio Francesco Retta
    Pages 1-18
  3. Tanmay S. Chavan, James O. Meyer, Luke Chisholm, Malgorzata Dobosz-Bartoszek, Vadim Gaponenko
    Pages 19-32
  4. Frederick D. Tsai, Joseph P. Wynne, Ian M. Ahearn, Mark R. Philips
    Pages 33-41
  5. Tripat Kaur Oberoi-Khanuja, Krishnaraj Rajalingam
    Pages 43-54
  6. Elisa Ferro, Luca Goitre, Eva Baldini, Saverio Francesco Retta, Lorenza Trabalzini
    Pages 55-74
  7. G. Aaron Hobbs, Harsha P. Gunawardena, Sharon L. Campbell
    Pages 75-96
  8. Elisa Ferro, Eva Baldini, Lorenza Trabalzini
    Pages 97-120
  9. Simon Schnaiter, Beatrix Fürst, Johannes Neu, Frigyes Wáczek, László Őrfi, György Kéri et al.
    Pages 121-129
  10. Putty-Reddy Sudhir, Jeou-Yuan Chen
    Pages 157-166
  11. Luca Goitre, Valentina Cutano, Saverio Francesco Retta
    Pages 197-205
  12. Lauren P. Huff, Molly J. DeCristo, Adrienne D. Cox
    Pages 263-283
  13. Annica K. B. Gad, Daniel Rönnlund, Jerker Widengren, Pontus Aspenström
    Pages 339-357
  14. Renata Tisi, Fiorella Belotti, Enzo Martegani
    Pages 359-390
  15. Enrico Bracco, Barbara Pergolizzi
    Pages 407-420
  16. G. Aaron Hobbs, Harsha P. Gunawardena, Sharon L. Campbell
    Pages E1-E1
  17. Back Matter
    Pages 421-425

About this book


Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, Ras Signaling: Methods and Protocols contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioral approaches, advanced high resolution fluorescence and electron microscopy imaging, and “omics” technologies.  Through this, the detailed volume provides information on expression, post-translational modifications, subcellular localization and dynamics, regulatory mechanisms of upstream and downstream signaling pathways, and, ultimately, biological activities and functions of RasGTPases in different model systems, including high and low eukaryotic organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Wide-ranging and authoritative, Ras Signaling: Methods and Protocols serves as an aid for investigators of different backgrounds and interests related to the multiple physiological and pathological functions of the large superfamily of RasGTPases.


Cell signaling GTP-binding and hydrolyzing proteins Model systems Pathological condition treatment Ras GTPase superfamily RasGTPases Signaling pathways

Editors and affiliations

  • Lorenza Trabalzini
    • 1
  • Saverio Francesco Retta
    • 2
  1. 1.University of SienaItaly
  2. 2.University of TorinoItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-790-7
  • Online ISBN 978-1-62703-791-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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