Optical Imaging of Neocortical Dynamics

  • Bruno Weber
  • Fritjof Helmchen

Part of the Neuromethods book series (NM, volume 85)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Fundamentals of Using Light to Study Neocortical Dynamics

    1. Front Matter
      Pages 1-1
    2. Fritjof Helmchen, Bruno Weber
      Pages 3-18
    3. Andrew K. Dunn
      Pages 33-51
    4. Walther Akemann, Hiroki Mutoh, Thomas Knöpfel
      Pages 53-72
    5. Lief E. Fenno, Karl Deisseroth
      Pages 73-86
  3. Shedding Light on Cortical Activity States

    1. Front Matter
      Pages 87-87
    2. Hongbo Jia, Arthur Konnerth
      Pages 89-105
    3. Masanori Murayama, Matthew E. Larkum
      Pages 107-116
    4. Carl C. H. Petersen
      Pages 117-132
    5. Benjamin F. Grewe, Fritjof Helmchen, Björn M. Kampa
      Pages 133-150
    6. David J. Margolis, Henry Lütcke, Fritjof Helmchen, Bruno Weber, Florent Haiss
      Pages 151-173
    7. Lief E. Fenno, Karl Deisseroth
      Pages 175-188
  4. Elucidating Metabolic States of Cortical Circuits

    1. Front Matter
      Pages 189-189
    2. Norio Takata, Yoshiaki Shinohara, Masamichi Ohkura, Tsuneko Mishima, Junichi Nakai, Hajime Hirase
      Pages 191-207
    3. Bianca Brawek, Olga Garaschuk
      Pages 209-223
    4. Anna Devor, Sava Sakadžić, Mohammad A. Yaseen, Emmanuel Roussakis, Peifang Tian, Hamutal Slovin et al.
      Pages 225-253
    5. Pavel Zakharov, Frank Scheffold, Bruno Weber
      Pages 255-271
    6. Andy Y. Shih, Jonathan D. Driscoll, Michael J. Pesavento, David Kleinfeld
      Pages 273-290
  5. Back Matter
    Pages 291-296

About this book


The application of optical methods for investigating neocortical circuit dynamics has greatly expanded in recent years, providing novel insights into the fascinating world of brain function. Optical Imaging of Neocortical Dynamics presents a guide to these indispensible tools, which cover a broad range of spatiotemporal scales and a  large variety of signaling aspects, ranging from voltage changes to metabolic states. This detailed volume specifically explores methods that are applied in experiments on living brains (in vivo) and that relate to functional properties on the spatial scale of cortical circuits.  Beginning with an introductory section that focuses on physical fundamentals of optical imaging as well as molecular tools used for in vivo optical imaging and optogenetic control, the book continues with the most relevant methods and their applications to investigate changes in neuronal and glial activity states as well as optical imaging methods probing metabolic states.  Written for the Neuromethods series, this volume contains the kind of detail and key implementation advice that ensures successful results in the lab.


Practical and easy to use, Optical Imaging of Neocortical Dynamics serves as an ideal guide for researchers who aim to apply these highly valuable tools to their own neuroscientific studies.


Cortical processing Functional optical imaging Glial activity states Neocortical circuit dynamics Neuronal activity states Optical probes Probing metabolic states

Editors and affiliations

  • Bruno Weber
    • 1
  • Fritjof Helmchen
    • 2
  1. 1.University of ZurichSwitzerland
  2. 2.University of ZurichSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-784-6
  • Online ISBN 978-1-62703-785-3
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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