Cell-Free Protein Synthesis

Methods and Protocols

  • Kirill Alexandrov
  • Wayne A. Johnston

Part of the Methods in Molecular Biology book series (MIMB, volume 1118)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Wayne A. Johnston, Kirill Alexandrov
    Pages 1-15
  3. Alexander A. Tokmakov, Atsushi Kurotani, Mikako Shirouzu, Yasuo Fukami, Shigeyuki Yokoyama
    Pages 17-33
  4. Aarthi Chandrasekaran, Anup K. Singh
    Pages 55-69
  5. Taichi E. Takasuka, Johnnie A. Walker, Lai F. Bergeman, Kirk A. Vander Meulen, Shin-ichi Makino, Nathaniel L. Elsen et al.
    Pages 71-95
  6. Christian Roos, Lei Kai, Stefan Haberstock, Davide Proverbio, Umesh Ghoshdastider, Yi Ma et al.
    Pages 109-130
  7. Sabrina Guillemer, Cécile Persillon, Jean-Michel Masson, Gilles Ravot
    Pages 131-147
  8. Tominari Kobayashi, Kodai Machida, Hiroaki Imataka
    Pages 149-156
  9. Kirsten Jackson, Ruba Khnouf, Z. Hugh Fan
    Pages 157-168
  10. Szilvia K. Nagy, Tamás Mészáros
    Pages 231-243
  11. Elizabeth A. Cook, Mingyue He
    Pages 245-255
  12. Tomomi Kimura-Soyema, Mikako Shirouzu, Shigeyuki Yokoyama
    Pages 267-273
  13. Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, Takuya Ueda
    Pages 275-284
  14. Toru Ezure, Takashi Suzuki, Eiji Ando
    Pages 285-296
  15. Xristo Zárate, David W. Galbraith
    Pages 297-307
  16. Kirill Alexandrov, Wayne A. Johnston
    Pages E1-E3
  17. Back Matter
    Pages 309-313

About this book


Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses.  Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies.  


biofuel enzymes cell-free enzyme expression cell-free methodology cell-free technology membrane proteins synthetic biology

Editors and affiliations

  • Kirill Alexandrov
    • 1
  • Wayne A. Johnston
    • 2
  1. 1.Institute for Molecular Bioscience (IMB)The University of QueenslandSt. LuciaAustralia
  2. 2.Institute for Molecular Bioscience (IMB)The University of QueenslandSt. LuciaAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-781-5
  • Online ISBN 978-1-62703-782-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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