Electron Microscopy

Methods and Protocols

  • John Kuo

Part of the Methods in Molecular Biology book series (MIMB, volume 1117)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Gregory M. Hendricks
    Pages 73-93
  3. Douglas E. Chandler, William P. Sharp
    Pages 95-132
  4. Andres Kaech, Urs Ziegler
    Pages 151-171
  5. Markus Grabenbauer, Hong-Mei Han, Jan Huebinger
    Pages 173-191
  6. Petr Chlanda, Martin Sachse
    Pages 193-214
  7. Margaret Coughlin, Aaron C. Groen, Timothy J. Mitchison
    Pages 259-271
  8. Christian Hacker, John M. Lucocq
    Pages 315-323
  9. Kenneth N. Goldie, Priyanka Abeyrathne, Fabian Kebbel, Mohamed Chami, Philippe Ringler, Henning Stahlberg
    Pages 325-341
  10. Dušan Cmarko, Anna Ligasová, Karel Koberna
    Pages 343-366
  11. Yuri L. Lyubchenko, Alexander A. Gall, Luda S. Shlyakhtenko
    Pages 367-384
  12. Wanzhong He, Yongning He
    Pages 445-483
  13. Coralie Spiegelhalter, Jocelyn F. Laporte, Yannick Schwab
    Pages 485-501
  14. Shigeki Watanabe, Martin Lehmann, Edward Hujber, Richard D. Fetter, Jackson Richards, Berit Söhl-Kielczynski et al.
    Pages 503-526
  15. Caroline Kizilyaprak, Anne Greet Bittermann, Jean Daraspe, Bruno M. Humbel
    Pages 541-558
  16. Heinz Schwarz, Bruno M. Humbel
    Pages 559-592
  17. Miriam S. Lucas, Maja Guenthert, Philippe Gasser, Falk Lucas, Roger Wepf
    Pages 593-616
  18. Clarissa Villinger, Martin Schauflinger, Heiko Gregorius, Christine Kranz, Katharina Höhn, Soufi Nafeey et al.
    Pages 617-638
  19. Godfried M. Roomans, Anca Dragomir
    Pages 639-661
  20. Martin Saunders, Jeremy A. Shaw
    Pages 689-706
  21. Matt R. Kilburn, Peta L. Clode
    Pages 733-755
  22. Back Matter
    Pages 783-799

About this book


This third edition of Electron Microscopy: Methods and Protocols  expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods.  With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation,  negative staining and immunogold labelling techniques,  DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy,  TEM crystallography and cryoTEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM , Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM, and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and NanoSIMS. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical,  Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.


(EM) data Cryo-specimen DNA and RNA tracking Focussed Ion Beam (FIB) Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) tomography bio-molecular electron microscopy bio-molecular research correlative microscopy cryoTEM 3D tomography electron microscopes

Editors and affiliations

  • John Kuo
    • 1
  1. 1.University of Western AustraliaCrawleyAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-775-4
  • Online ISBN 978-1-62703-776-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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