Gene Correction

Methods and Protocols

  • Francesca Storici

Part of the Methods in Molecular Biology book series (MIMB, volume 1114)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Approaches for Gene Correction from Bacteria to Human Cells

    1. Front Matter
      Pages 1-1
    2. Akira Motegi, Minoru Takata
      Pages 25-35
  3. Gene Correction Tools Using Non-Viral or Viral Systems

  4. Breaking the DNA to Stimulate Gene Correction: Nuclease Design, In Vivo Test, Efficacy, and Off-Target Effect

    1. Front Matter
      Pages 201-201
    2. Yanni Lin, Thomas J. Cradick, Gang Bao
      Pages 203-219
    3. Luhan Yang, Prashant Mali, Caroline Kim-Kiselak, George Church
      Pages 245-267
    4. Neena K. Pyzocha, F. Ann Ran, Patrick D. Hsu, Feng Zhang
      Pages 269-277
    5. Matthew L. Hirsch, R. Jude Samulski
      Pages 291-307
    6. Céline J. Rocca, Hayder H. Abdul-Razak, Michael C. Holmes, Philip D. Gregory, Rafael J. Yáñez-Muñoz
      Pages 325-338
  5. Gene Correction Approaches for Specific Disease Models

    1. Front Matter
      Pages 385-385
    2. Marilyn Dernigoghossian, Arthur Krigel, Francine Behar-Cohen, Charlotte Andrieu-Soler
      Pages 387-398
    3. Lingzhi Cai, Bhanu Munil Koppanati, Carmen Bertoni, Paula R. Clemens
      Pages 399-411
    4. Alexandra Sherman, Alexander Schlachterman, Mario Cooper, Elizabeth P. Merricks, Robin A. Raymer, Dwight A. Bellinger et al.
      Pages 413-426
  6. Back Matter
    Pages 451-463

About this book


Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy.


gene correction gene repair gene targeting human genome human pluripotent stem cells

Editors and affiliations

  • Francesca Storici
    • 1
  1. 1.Georgia Institute of TechnologyAtlantaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-760-0
  • Online ISBN 978-1-62703-761-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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