Artificial Riboswitches

Methods and Protocols

  • Atsushi Ogawa

Part of the Methods in Molecular Biology book series (MIMB, volume 1111)

Table of contents

  1. Front Matter
    Pages i-x
  2. Yaroslav G. Chushak, Jennifer A. Martin, Jorge L. Chávez, Nancy Kelley-Loughnane, Morley O. Stone
    Pages 1-15
  3. Asako Murata, Shin-ichi Sato
    Pages 17-28
  4. Gosuke Hayashi, Kazuhiko Nakatani
    Pages 29-40
  5. Zohaib Ghazi, Casey C. Fowler, Yingfu Li
    Pages 57-75
  6. Svetlana V. Harbaugh, Molly E. Chapleau, Yaroslav G. Chushak, Morley O. Stone, Nancy Kelley-Loughnane
    Pages 77-91
  7. Ye Jin, Jian-Dong Huang
    Pages 93-105
  8. Helen A. Vincent, Christopher J. Robinson, Ming-Cheng Wu, Neil Dixon, Jason Micklefield
    Pages 107-129
  9. Yoko Nomura, Yohei Yokobayashi
    Pages 131-140
  10. Kohei Ike, Daisuke Umeno
    Pages 141-152
  11. Atsushi Ogawa
    Pages 165-181
  12. Kei Endo, Hirohide Saito
    Pages 183-196
  13. Kazuhiro Furukawa, Hongzhou Gu, Ronald R. Breaker
    Pages 209-220
  14. Jonathan A. Goler, James M. Carothers, Jay D. Keasling
    Pages 221-235
  15. Charlotte Rehm, Jörg S. Hartig
    Pages 237-249
  16. Chase L. Beisel, Ryan J. Bloom, Christina D. Smolke
    Pages 259-267
  17. Shunnichi Kashida, Hirohide Saito
    Pages 269-286
  18. Back Matter
    Pages 287-289

About this book


Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protocols focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, protocols for evaluating the activities of the resultant riboswitches, as well as protocols for construction of ligand-dependent, trans-acting gene regulators. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Artificial Riboswitches: Methods and Protocols seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field.


RNA aptamers artificial riboswitches ligand-responsive gene regulator recombinant proteins synthetic riboswitches

Editors and affiliations

  • Atsushi Ogawa
    • 1
  1. 1.Ehime UniversityMatsuyamaJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-754-9
  • Online ISBN 978-1-62703-755-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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