© 2014

Optimization in Drug Discovery

In Vitro Methods

  • Gary W. Caldwell
  • Zhengyin Yan
  • Provides updated variations on assays as well as numerous new protocols for drug discovery optimization

  • Features readily reproducible methods and protocols that are lab-tested and successful

  • Includes expert tips and key implementation advice


Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Gary W. Caldwell, Becki Hasting, John A. Masucci, Zhengyin Yan
    Pages 1-20
  3. Dennis Kalamaridis, Nayan Patel
    Pages 21-37
  4. Dennis Kalamaridis, Karen DiLoreto
    Pages 39-47
  5. Gary W. Caldwell, Chrissa Ferguson, Robyn Buerger, Lovonia Kulp, Zhengyin Yan
    Pages 49-76
  6. Maria Markowska, L. Mark Kao
    Pages 77-86
  7. Kevin J. Coe, Tatiana Koudriakova
    Pages 87-99
  8. Carlo Sensenhauser
    Pages 137-169
  9. Caitlin Lynch, Haishan Li, Hongbing Wang
    Pages 195-205
  10. Guochun He, Jing Zhao, Jennifer C. Brennan, Alessandra A. Affatato, Bin Zhao, Robert H. Rice et al.
    Pages 221-235
  11. Monica Singer, Carlo Sensenhauser, Shannon Dallas
    Pages 237-253
  12. Kevin J. Coe, Judith Skaptason, Tatiana Koudriakova
    Pages 305-314

About this book


Thoroughly revised and updated, Optimization in Drug Discovery: In Vitro Methods, Second Edition presents a wide spectrum of in vitro assays including formulation, plasma binding, absorption and permeability, cytochrome P450 (CYP) and UDP-glucuronosyltransferases (UGT) metabolism, CYP inhibition and induction, drug transporters, drug-drug interactions via assessment of reactive metabolites, genotoxicity, and chemical and photo-mutagenicity assays. Written for the Methods in Pharmacology and Toxicology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Expert authors have developed and utilized these in vitro assays to achieve “drug-like” characteristics in addition to efficacy properties and good safety profiles of drug candidates.


Comprehensive and up-to-date, Optimization in Drug Discovery: In Vitro Methods, Second Edition aims to guide researchers down the difficult path to successful drug discovery and development.


Biopharmaceutics Drug candidates In vitro and in situ assays Pharmacodynamic Pharmacokinetics Toxicokinetics

Editors and affiliations

  • Gary W. Caldwell
    • 1
  • Zhengyin Yan
    • 2
  1. 1.CREATe Core Science TechnologiesJanssen Research & Development, LLCSpring HouseUSA
  2. 2.CREATe Core Science TechnologiesJanssen Research & Development, LLCSpring HouseUSA

Bibliographic information

  • Book Title Optimization in Drug Discovery
  • Book Subtitle In Vitro Methods
  • Editors Gary W. Caldwell
    Zhengyin Yan
  • Series Title Methods in Pharmacology and Toxicology
  • Series Abbreviated Title Meth.Pharmacology,Toxicology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-62703-741-9
  • Softcover ISBN 978-1-4939-6070-5
  • eBook ISBN 978-1-62703-742-6
  • Series ISSN 1557-2153
  • Series E-ISSN 1940-6053
  • Edition Number 2
  • Number of Pages XVII, 597
  • Number of Illustrations 64 b/w illustrations, 49 illustrations in colour
  • Topics Pharmacology/Toxicology
  • Buy this book on publisher's site
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