The Complement System

Methods and Protocols

  • Mihaela Gadjeva

Part of the Methods in Molecular Biology book series (MIMB, volume 1100)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Mihaela Gadjeva
    Pages 1-9
  3. Anja Roos, Jörgen Wieslander
    Pages 11-23
  4. Véronique Rossi, Isabelle Bally, Monique Lacroix, Gérard J. Arlaud, Nicole M. Thielens
    Pages 43-60
  5. Angela W. Norton, Paolo G. V. Martini, Lynette C. Cook, Scott Alderucci, Dianna M. Lundberg, Susan M. Fish et al.
    Pages 61-74
  6. Marieta M. Ruseva, Meike Heurich
    Pages 75-91
  7. Lars Sottrup-Jensen, Gregers Rom Andersen
    Pages 93-102
  8. Oren Moskovich, Zvi Fishelson
    Pages 103-108
  9. Lea Munthe-Fog, Hans Ole Madsen, Peter Garred
    Pages 123-130
  10. Misao Matsushita, David Kilpatrick, Hiroshi Shiraki, Yu Liu, Koichiro Tateishi, Mitsushi Tsujimura et al.
    Pages 141-159
  11. Joseph O’Flynn, Maria C. Faber Krol, Mohamed R. Daha, Cees van Kooten
    Pages 161-167
  12. Sara C. Nilsson, Anna M. Blom
    Pages 177-188
  13. Lilian Varga, József Dobó
    Pages 189-205
  14. Bing-Bin Yu, Beryl E. Moffatt, Marina Fedorova, Claire G. S. Villiers, James N. Arnold, Eugenie Du et al.
    Pages 207-223
  15. Mihály Józsi, Seppo Meri
    Pages 225-236
  16. Lubka T. Roumenina, Roxane Roquigny, Caroline Blanc, Nelly Poulain, Stéphanie Ngo, Marie-Agnès Dragon-Durey et al.
    Pages 237-247
  17. Marie-Agnès Dragon-Durey, Caroline Blanc, Lubka T. Roumenina, Nelly Poulain, Stéphanie Ngo, Pauline Bordereau et al.
    Pages 249-256
  18. Najmunisa Karbani, Eswari Dodagatta-Marri, Asif S. Qaseem, Priyaa Madhukaran, Patrick Waters, Anthony G. Tsolaki et al.
    Pages 257-272
  19. Eswari Dodagatta-Marri, Asif S. Qaseem, Najmunisa Karbani, Anthony G. Tsolaki, Patrick Waters, Taruna Madan et al.
    Pages 273-290
  20. Christian M. Karsten, Yves Laumonnier, Jörg Köhl
    Pages 291-304
  21. Berhane Ghebrehiwet, Ellinor I. B. Peerschke
    Pages 319-327
  22. Martin Kolev, Claudia Kemper
    Pages 329-339
  23. Martin Kolev
    Pages 341-346
  24. Gitte Krogh Nielsen, Thomas Vorup-Jensen
    Pages 355-364
  25. Nirmal K. Banda, Kazue Takahashi
    Pages 365-371
  26. Markryan Dwyer, Mihaela Gadjeva
    Pages 373-379
  27. Back Matter
    Pages 381-383

About this book


Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols  to purify and analyze the activity of the individual complement components or pathways.  It includes assays that describe detection  of complement SNPs,  clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established “classical” assays and recently developed “new” assays to analyze the complement system activation will be useful to a wide audience of scientists.


activation in humans activation in model animals complement system complement system activation disease health homeostasis immune surveillance methodological aspects receptors soluble components

Editors and affiliations

  • Mihaela Gadjeva
    • 1
  1. 1.Division of Infectious Diseases, Brigham and Women’s HospitalHarvard University Harvard Medical SchoolBostonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-723-5
  • Online ISBN 978-1-62703-724-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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