Bioluminescent Imaging

Methods and Protocols

  • Christian E. Badr

Part of the Methods in Molecular Biology book series (MIMB, volume 1098)

Table of contents

  1. Front Matter
    Pages i-xi
  2. In Vitro Bioassays

    1. Front Matter
      Pages 19-19
    2. M. Hannah Degeling, M. Sarah S. Bovenberg, Marie Tannous, Bakhos A. Tannous
      Pages 47-55
  3. Imaging Molecular and Cellular Events

    1. Front Matter
      Pages 57-57
    2. Kathryn E. Luker, Gary D. Luker
      Pages 59-69
    3. Hae Young Ko, Young Sik Lee, Soonhag Kim
      Pages 85-95
    4. Miguel A. S. Cavadas, Alex Cheong
      Pages 97-105
    5. Dan Close, Tingting Xu, Steven Ripp, Gary Sayler
      Pages 107-116
  4. Imaging of Disease (Pathogenesis)

    1. Front Matter
      Pages 127-127
    2. Hiroshi Yamashita, Dan T. Nguyen, Euiheon Chung
      Pages 145-151
    3. Greetje Vande Velde, Soňa Kucharíková, Patrick Van Dijck, Uwe Himmelreich
      Pages 153-167
    4. Jonathan M. Warawa, Matthew B. Lawrenz
      Pages 169-181
  5. Therapeutic Imaging

    1. Front Matter
      Pages 183-183
    2. Romain J. Amante, Christian E. Badr
      Pages 185-195
  6. Emerging Technologies

    1. Front Matter
      Pages 229-229
    2. Charles P. Lai, Bakhos A. Tannous, Xandra O. Breakefield
      Pages 249-258
    3. Joe Dragavon, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson, Samantha Blazquez, Kelly L. Rogers et al.
      Pages 259-270
  7. Back Matter
    Pages 271-276

About this book


Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly).  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.


Aequorin Bioluminescence imaging Bioluminescent reporters Firefly luciferase Gaussia luciferase Imaging of disease In vitro bioassays In vivo imaging Luciferase Luciferin

Editors and affiliations

  • Christian E. Badr
    • 1
  1. 1.Department of Neurology, Neuroscience CenterMassachusetts General HospitalBostonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-717-4
  • Online ISBN 978-1-62703-718-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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