miRNA Maturation

Methods and Protocols

  • Christoph Arenz

Part of the Methods in Molecular Biology book series (MIMB, volume 1095)

Table of contents

  1. Front Matter
    Pages i-x
  2. Reviews

    1. Front Matter
      Pages 1-1
    2. Michael Sand
      Pages 3-10
    3. Marlen Hesse, Christoph Arenz
      Pages 11-25
    4. Valerie T. Tripp, Jaclyn R. McKenna, Douglas D. Young
      Pages 27-58
  3. Methods

    1. Front Matter
      Pages 59-59
    2. Vera Bilan, Danilo Allegra, Florian Kuchenbauer, Daniel Mertens
      Pages 87-93
    3. Marlen Hesse, Brian P. Davies, Christoph Arenz
      Pages 95-102
    4. Hannah Zöllner, Stephan A. Hahn, Abdelouahid Maghnouj
      Pages 121-134
    5. Colleen M. Connelly, Alexander Deiters
      Pages 135-146
    6. Colleen M. Connelly, Alexander Deiters
      Pages 147-156
    7. Concetta Avitabile, Enrica Fabbri, Nicoletta Bianchi, Roberto Gambari, Alessandra Romanelli
      Pages 157-164
    8. Eleonora Brognara, Enrica Fabbri, Nicoletta Bianchi, Alessia Finotti, Roberto Corradini, Roberto Gambari
      Pages 165-176
    9. Hannah Zöllner, Stephan A. Hahn, Abdelouahid Maghnouj
      Pages 177-190
  4. Back Matter
    Pages 191-192

About this book


In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.


Dicer-mediated miRNA maturation Drosha activity PNA as anti-miRs biological mediator fluorescent methods manipulate miRNA activity miRNA maturation inhibitors non-fluorescent methods oligonucleotide approaches potential drug target qRT-PCR of miRNA maturation components small molecule miRNA effectors

Editors and affiliations

  • Christoph Arenz
    • 1
  1. 1.Institute for ChemistryHumboldt Universität zu BerlinBerlinGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-702-0
  • Online ISBN 978-1-62703-703-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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