Structural Genomics

General Applications

  • Yu Wai Chen

Part of the Methods in Molecular Biology book series (MIMB, volume 1091)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Cloning, Expression and Protein Production

    1. Front Matter
      Pages 1-1
    2. Yuanpeng Janet Huang, Thomas B. Acton, Gaetano T. Montelione
      Pages 3-16
    3. Claire Strain-Damerell, Pravin Mahajan, Opher Gileadi, Nicola A. Burgess-Brown
      Pages 55-72
    4. Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Opher Gileadi, Susanne Gräslund
      Pages 73-94
    5. Pravin Mahajan, Claire Strain-Damerell, Opher Gileadi, Nicola A. Burgess-Brown
      Pages 95-121
    6. Deepak B. Thimiri Govinda Raj, Lakshmi S. Vijayachandran, Imre Berger
      Pages 123-130
    7. Yan Nie, Itxaso Bellon-Echeverria, Simon Trowitzsch, Christoph Bieniossek, Imre Berger
      Pages 131-141
    8. Susan D. Chapple, Michael R. Dyson
      Pages 143-149
    9. Takaho Terada, Takeshi Murata, Mikako Shirouzu, Shigeyuki Yokoyama
      Pages 151-159
    10. Shin-ichi Makino, Emily T. Beebe, John L. Markley, Brian G. Fox
      Pages 161-178
    11. Ben Chisnall, Courtney Johnson, Yavuz Kulaberoglu, Yu Wai Chen
      Pages 179-186
  3. Experimental Structure Determination and Characterization

    1. Front Matter
      Pages 187-187
    2. Vincent Mariaule, Florine Dupeux, José A. Márquez
      Pages 189-195
    3. Yunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick, Michael A. Kennedy
      Pages 215-227
    4. Elodie Crublet, Rime Kerfah, Guillaume Mas, Marjolaine Noirclerc-Savoye, Violaine Lantez, Thierry Vernet et al.
      Pages 229-244
    5. Kevin N. Dyer, Michal Hammel, Robert P. Rambo, Susan E. Tsutakawa, Ivan Rodic, Scott Classen et al.
      Pages 245-258
  4. Computational Methods and Structural Data Analyses

    1. Front Matter
      Pages 275-275
    2. Benjamin Webb, Keren Lasker, Javier Velázquez-Muriel, Dina Schneidman-Duhovny, Riccardo Pellarin, Massimiliano Bonomi et al.
      Pages 277-295
    3. Marcin J. Domagalski, Heping Zheng, Matthew D. Zimmerman, Zbigniew Dauter, Alexander Wlodawer, Wladek Minor
      Pages 297-314
    4. Andrei L. Turinsky, Sabry Razick, Brian Turner, Ian M. Donaldson, Shoshana J. Wodak
      Pages 315-331
    5. Marjorie M. Harding, Kun-Yi Hsin
      Pages 333-342
  5. Back Matter
    Pages 353-358

About this book


The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.


macromolecular machinery protein production recombinant human proteins structural biology structural studies

Editors and affiliations

  • Yu Wai Chen
    • 1
  1. 1.King's College LondonLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-690-0
  • Online ISBN 978-1-62703-691-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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