Plant Metabolic Flux Analysis

Methods and Protocols

  • Martine Dieuaide-Noubhani
  • Ana Paula Alonso

Part of the Methods in Molecular Biology book series (MIMB, volume 1090)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Martine Dieuaide-Noubhani, Ana Paula Alonso
    Pages 1-17
  3. Marie Beurton-Aimar, Tung Vu-Ngoc Nguyen, Sophie Colombié
    Pages 19-38
  4. Experimental Design and Acquistion of Labeling Data (Experimental Design and Data Acquisition for Isotopic Labeling Based Metabolic Flux Analysis)

    1. Front Matter
      Pages 39-39
    2. Katia Pianelli, Antoine Monier, Marie-Hélène Andrieu, Bertrand Beauvoit, Martine Dieuaide-Noubhani
      Pages 41-52
    3. Nicholas J. Kruger, Shyam K. Masakapalli, R. George Ratcliffe
      Pages 53-72
    4. Shyam K. Masakapalli, R. George Ratcliffe, Thomas C. R. Williams
      Pages 73-86
    5. Quyen Truong, Jacqueline V. Shanks
      Pages 87-105
    6. Wagner L. Araújo, Takayuki Tohge, Adriano Nunes-Nesi, Toshihiro Obata, Alisdair R. Fernie
      Pages 107-119
    7. Mohamed Koubaa, Brigitte Thomasset, Albrecht Roscher
      Pages 121-130
    8. Albrecht Roscher, Stéphanie Troufflard, Abdelghani Idrissi Taghki
      Pages 143-152
  5. From Labeling Data to Flux Maps (from the Labeling Data to a Metabolic Map)

    1. Front Matter
      Pages 153-153
    2. Lara J. Jazmin, John P. O’Grady, Fangfang Ma, Doug K. Allen, John A. Morgan, Jamey D. Young
      Pages 181-210
    3. Amy Marshall-Colon, Neelanjan Sengupta, David Rhodes, John A. Morgan
      Pages 211-222
    4. C. Hart Poskar, Jan Huege, Christian Krach, Yair Shachar-Hill, Björn H. Junker
      Pages 223-246
  6. Predicting Fluxes in Plants

    1. Front Matter
      Pages 247-247
    2. Guillaume Ménard, Benoit Biais, Duyên Prodhomme, Patricia Ballias, Yves Gibon
      Pages 249-259
    3. Gilles Curien, María L. Cárdenas, Athel Cornish-Bowden
      Pages 261-280
    4. Eva Grafahrend-Belau, Astrid Junker, Falk Schreiber, Björn H. Junker
      Pages 281-299
    5. Cristiana G. O. Dal’Molin, Lake-Ee Quek, Robin W. Palfreyman, Lars K. Nielsen
      Pages 317-332
  7. Flux Analysis at the Whole Plant Level

    1. Front Matter
      Pages 333-333
    2. Christophe Salon, Marie-Paule Bataillé, Karine Gallardo, Christian Jeudy, Anne-Lise Santoni, Jacques Trouverie et al.
      Pages 335-346
    3. Valentina Baldazzi, Nadia Bertin, Hélène Gautier, Michel Génard
      Pages 347-361
  8. Back Matter
    Pages 363-365

About this book


In Plant Metabolic Flux Analysis, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.




Authoritative and practical Plant Metabolic Flux Analysis,  seeks to aid scientists in the further study of cutting-edge protocols and methodologies that are crucial to getting ahead in MFA.


Metabolic Flux analysis (MFA) biomass synthesis flux quantification workflow metabolic engineering metabolic networks metabolism predictive modeling

Editors and affiliations

  • Martine Dieuaide-Noubhani
    • 1
  • Ana Paula Alonso
    • 2
  1. 1.INRA Bordeaux Université Bordeaux SegalenVillenave d'OrnonFrance
  2. 2.Department of Molecular GeneticsThe Ohio State UniversityColumbusUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-687-0
  • Online ISBN 978-1-62703-688-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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