Methods and Protocols

  • Miguel Chillón
  • Assumpció Bosch

Part of the Methods in Molecular Biology book series (MIMB, volume 1089)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Rosa Menéndez-Conejero, Ana J. Pérez-Berná, Gabriela N. Condezo, Alvaro Ortega-Esteban, Marta del Alamo, Pedro J. de Pablo et al.
    Pages 1-24
  3. Sara Bergström Lind, Konstantin A. Artemenko, Ulf Pettersson
    Pages 25-44
  4. Alan L. Parker, Angela C. Bradshaw, Raul Alba, Stuart A. Nicklin, Andrew H. Baker
    Pages 45-59
  5. Charleine Zussy, Sara Salinas
    Pages 71-78
  6. Stefania Piersanti, Enrico Tagliafico, Isabella Saggio
    Pages 89-104
  7. Thomas Speiseder, Michael Nevels, Thomas Dobner
    Pages 105-115
  8. Raul Gil-Hoyos, Juan Miguel-Camacho, Ramon Alemany
    Pages 117-132
  9. Nelson C. Di Paolo, Dmitry M. Shayakhmetov
    Pages 133-141
  10. Zsolt Ruzsics, Frederic Lemnitzer, Christian Thirion
    Pages 143-158
  11. Susana Miravet, Maria Ontiveros, Jose Piedra, Cristina Penalva, Mercè Monfar, Miguel Chillón
    Pages 159-173
  12. Ana Carina Silva, Paulo Fernandes, Marcos F. Q. Sousa, Paula M. Alves
    Pages 175-196
  13. Meritxell Puig, Jose Piedra, Susana Miravet, María Mercedes Segura
    Pages 197-210
  14. Florian Kreppel
    Pages 211-229
  15. Marta Miralles, Marc Garcia, Marcos Tejero, Assumpció Bosch, Miguel Chillón
    Pages 231-243
  16. Back Matter
    Pages 245-247

About this book


Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics.  Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.


Adenoviral particles Host cell interactions Human Ad5 Non-human adenovirus vectors Virology research

Editors and affiliations

  • Miguel Chillón
    • 1
  • Assumpció Bosch
    • 2
  1. 1.Biochemistry and Molecular BiologyUniversitat Autònoma de BarcelonaBellaterraSpain
  2. 2.Biochemistry and Molecular BiologyUniversitat Autònoma de BarcelonaBellaterraSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-678-8
  • Online ISBN 978-1-62703-679-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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