Methods and Protocols

  • Matthew D. Hirschey

Part of the Methods in Molecular Biology book series (MIMB, volume 1077)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Methods to Generate Sirtuin Biology Tools

    1. Front Matter
      Pages 1-1
    2. Leonard Guarente
      Pages 3-10
    3. Scott Tsuchiyama, Elizabeth Kwan, Weiwei Dang, Antonio Bedalov, Brian K. Kennedy
      Pages 11-37
    4. Mohan Viswanathan, Heidi A. Tissenbaum
      Pages 39-56
    5. Jason G. Wood, Rachel Whitaker, Stephen L. Helfand
      Pages 57-67
    6. Kathleen A. Hershberger, Jonathan Motley, Matthew D. Hirschey, Kristin A. Anderson
      Pages 69-78
  3. Methods to Identify Sirtuin Substrates

    1. Front Matter
      Pages 79-79
    2. Yu Li, Jeffrey C. Silva, Mary E. Skinner, David B. Lombard
      Pages 81-104
    3. Yue Chen, Gozde Colak, Yingming Zhao
      Pages 105-120
    4. Matthew J. Rardin, Jason M. Held, Bradford W. Gibson
      Pages 121-131
    5. J. Will Thompson, Alex Robeson, Joshua L. Andersen
      Pages 133-148
    6. Lei Zhong, Barbara Martinez-Pastor, Dafne M. Silberman, Carlos Sebastian, Raul Mostoslavsky
      Pages 149-163
  4. Methods to Measure Sirtuin Activity

  5. Methods to Study Sirtuin Biology

    1. Front Matter
      Pages 239-239
    2. Dongning Wang, Michelle F. Green, Eoin McDonnell, Matthew D. Hirschey
      Pages 241-258
    3. In Hye Lee, Jeanho Yun, Toren Finkel
      Pages 259-271
    4. Paloma Martínez-Redondo, Alejandro Vaquero
      Pages 273-283
    5. Kathryn Moynihan Ramsey, Alison H. Affinati, Clara B. Peek, Biliana Marcheva, Hee-Kyung Hong, Joseph Bass
      Pages 285-302
  6. Matthew D. Hirschey
    Pages E1-E1
  7. Back Matter
    Pages 313-314

About this book


Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and easy to use, Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology.


Biological pathways Gene family Model organisms NAD(+)-dependent protein deacylases Sirtuin substrates

Editors and affiliations

  • Matthew D. Hirschey
    • 1
  1. 1.Duke University Medical Center Institute of Molecular PhysiologyDurhamUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-636-8
  • Online ISBN 978-1-62703-637-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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