© 2013

Hormone Assays in Biological Fluids

  • Michael J. Wheeler

Part of the Methods in Molecular Biology book series (MIMB, volume 1065)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Michael J. Wheeler
    Pages 1-6
  3. Michael J. Wheeler
    Pages 7-25
  4. Alberto Sánchez-Guijo, Michaela F. Hartmann, Stefan A. Wudy
    Pages 27-44
  5. Michael J. Wheeler
    Pages 105-116
  6. Nadia El-Farhan, David Hampton, Michael Penney
    Pages 129-139
  7. Michael J. Wheeler
    Pages 141-146
  8. Steve Bloom, Mohammad Ghatei, Paul Bech
    Pages 147-170
  9. Helen P. Field, Michael J. Wheeler
    Pages 211-226
  10. Sophie C. Barnes
    Pages 227-234
  11. Sophie C. Barnes
    Pages 235-244
  12. Loretta Ford
    Pages 245-257
  13. Norman F. Taylor
    Pages 259-276
  14. Edward Kearney
    Pages 277-289
  15. Catharine Sturgeon
    Pages 291-305

About this book


Hormone measurement is necessary for the diagnosis of a wide range of clinical conditions and is essential for monitoring the effectiveness of treatment. As the number of hormone requests in the clinical field rises exponentially, it has become imperative to create hormone assays accessible to researchers with a varied range of equipment. Hormone Assays in Biological Fluids, Second Edition reviews common techniques used to measure hormones as well as relatively new methods such as tandem mass spectrometry. Additionally, subsequent chapters detail methods for a broad range of hormones; Techniques and principles covered are transferable to a wide range of substances across species. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Hormone Assays in Biological Fluids, Second Edition will serve students, technologists, laboratory scientists, and researchers looking to apply, or attain a greater understanding of, methods for measuring hormones.


endocrinology gas chromatography hormone measurement immunoassays tandem mass spectrometry

Editors and affiliations

  • Michael J. Wheeler
    • 1
  1. 1.Consultant Clinical Scientist The Old DairyExmouthUnited Kingdom

Bibliographic information

  • Book Title Hormone Assays in Biological Fluids
  • Editors Michael J. Wheeler
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-62703-615-3
  • Softcover ISBN 978-1-4939-6280-8
  • eBook ISBN 978-1-62703-616-0
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XI, 310
  • Number of Illustrations 20 b/w illustrations, 14 illustrations in colour
  • Topics Molecular Medicine
  • Buy this book on publisher's site
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