Legume Genomics

Methods and Protocols

  • Ray J. Rose

Part of the Methods in Molecular Biology book series (MIMB, volume 1069)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Steven B. Cannon
    Pages 1-14
  3. Shaun J. Curtin, Justin E. Anderson, Colby G. Starker, Nicholas J. Baltes, Dhananjay Mani, Daniel F. Voytas et al.
    Pages 25-42
  4. Rohini Garg, Mukesh Jain
    Pages 43-58
  5. Nicolas Goffard, Georg Weiller
    Pages 59-66
  6. Cecilia Contreras-Cubas, Alejandra A. Covarrubias, José Luis Reyes
    Pages 81-92
  7. Juan Carlos Serrani Yarce, Hee-Kyung Lee, Million Tadege, Pascal Ratet, Kirankumar S. Mysore
    Pages 93-100
  8. Maria Carelli, Ornella Calderini, Francesco Panara, Andrea Porceddu, Ilaria Losini, Pietro Piffanelli et al.
    Pages 101-118
  9. Dorian Fabian Urbański, Anna Małolepszy, Jens Stougaard, Stig Uggerhøj Andersen
    Pages 119-146
  10. James L. Weller, Valérie F. G. Hecht, Frances C. Sussmilch
    Pages 147-161
  11. Daniela S. Floss, Alexa M. Schmitz, Colby G. Starker, J. Stephen Gantt, Maria J. Harrison
    Pages 163-177
  12. Kelsey Picard, Robyn Lee, Roger Hellens, Richard Macknight
    Pages 215-226
  13. Mark A. Baker
    Pages 271-277
  14. Amit Rai, Shivshankar Umashankar, Sanjay Swarup
    Pages 279-312
  15. Back Matter
    Pages 313-316

About this book


Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.


Genome characterization and analysis Medicago and Lotus Model legumes Molecular breeders Molecular markers Plant-microbe interactions Stable plant transformation Transient expression

Editors and affiliations

  • Ray J. Rose
    • 1
  1. 1.CILRThe University of Newcastle Environmental & Life SciencesCallaghanAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-612-2
  • Online ISBN 978-1-62703-613-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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