Human Monoclonal Antibodies

Methods and Protocols

  • Michael Steinitz

Part of the Methods in Molecular Biology book series (MIMB, volume 1060)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sotirios Kotsovilis, Evangelos Andreakos
    Pages 37-59
  3. Jamie Jarboe, Anumeha Gupta, Wasif Saif
    Pages 61-77
  4. Bharath Wootla, Aleksandar Denic, Moses Rodriguez
    Pages 79-110
  5. T. Kuramochi, T. Igawa, H. Tsunoda, K. Hattori
    Pages 123-137
  6. Kohei Kurosawa, Waka Lin, Kunihiro Ohta
    Pages 139-148
  7. Jarrett J. Adams, Bryce Nelson, Sachdev S. Sidhu
    Pages 149-170
  8. André Frenzel, Jonas Kügler, Sonja Wilke, Thomas Schirrmann, Michael Hust
    Pages 215-243
  9. Susana Magadán Mompó, África González-Fernández
    Pages 245-276
  10. Cecilia Deantonio, Diego Cotella, Paolo Macor, Claudio Santoro, Daniele Sblattero
    Pages 277-295
  11. Vladimir Tolmachev, Anna Orlova, Karl Andersson
    Pages 309-330
  12. Thomas Müller-Späth, Massimo Morbidelli
    Pages 331-351
  13. Miri Blank, Tomer Bashi, Yehuda Shoenfeld
    Pages 353-361
  14. Michael Steinitz
    Pages E1-E1
  15. Back Matter
    Pages 363-364

About this book


The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies, as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting.


antibody immunogenicity chimeric antibodies gene libraries human monoclonal antibodies polyclonal antibodies

Editors and affiliations

  • Michael Steinitz
    • 1
  1. 1.Department of PathologyThe Hebrew University Hadassah Medical SchoolJerusalemIsrael

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-585-9
  • Online ISBN 978-1-62703-586-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals