Nucleic Acid Detection

Methods and Protocols

  • Dmitry M. Kolpashchikov
  • Yulia V. Gerasimova

Part of the Methods in Molecular Biology book series (MIMB, volume 1039)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Advances in Sample Preparation

  3. New Real-Time and Instantaneous Assays

    1. Front Matter
      Pages 29-29
    2. Elisa Mokany, Alison V. Todd
      Pages 31-49
    3. Fang Liang, Neetika Arora, Kang Liang Zhang, David Che Cheng Yeh, Richard Lai, Darnley Pearson et al.
      Pages 51-68
    4. Yulia V. Gerasimova, Jack Ballantyne, Dmitry M. Kolpashchikov
      Pages 69-80
    5. Evan M. Cornett, Dmitry M. Kolpashchikov
      Pages 81-86
  4. Isothermal Amplification Methods for Nucleic Acid Detection

    1. Front Matter
      Pages 87-87
    2. Shouyi Chen, Xunde Li, Juntao Li, Edward R. Atwill
      Pages 99-108
    3. Yasuhito Sako, Agathe Nkouawa, Tetsuya Yanagida, Akira Ito
      Pages 109-120
    4. Hiroka Aonuma, Athanase Badolo, Kiyoshi Okado, Hirotaka Kanuka
      Pages 121-127
  5. Signal Amplification Approach

    1. Front Matter
      Pages 129-129
    2. Bingjie Zou, Yinjiao Ma, Guohua Zhou
      Pages 131-137
  6. Non-fluorescent Detection Formats

    1. Front Matter
      Pages 139-139
    2. Lingwen Zeng, Puchang Lie, Zhiyuan Fang, Zhuo Xiao
      Pages 161-167
    3. Mei-Sheng Wu, Guang-sheng Qian, Jing-Juan Xu, Hong-Yuan Chen
      Pages 169-179
    4. Kumarasamy Jayakumar, Rajendiran Rajesh, Venkataraman Dharuman, Rangarajan Venkatesan
      Pages 201-219
  7. Advances in FISH

    1. Front Matter
      Pages 221-221
    2. Evgeniy S. Morozkin, Tatyana V. Karamysheva, Pavel P. Laktionov, Valentin V. Vlassov, Nikolay B. Rubtsov
      Pages 233-242
  8. Detection of MicroRNA

    1. Front Matter
      Pages 243-243
    2. Wenhong Tian, Xiaoyan Dong, Xiaobing Wu, Zhijian Wu
      Pages 261-277
    3. Meredith B. Baker, Gang Bao, Charles D. Searles
      Pages 279-287
  9. RNA Imaging in Live Cells

    1. Front Matter
      Pages 289-289
    2. Susann Kummer, Andrea Knoll, Andreas Herrmann, Oliver Seitz
      Pages 291-301
  10. Back Matter
    Pages 319-320

About this book


In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.


DNA DNA for amplification and analysis RNA RNA monitoring in live cells capillary electrophoresis fluorescent in situ hybridization isothermal amplification techniques microarrays molecular beacon probes for micro RNA analysis nucleic acid detection methods point-of care diagnostics qPCR chemistries signal amplification technique visual or electric signals

Editors and affiliations

  • Dmitry M. Kolpashchikov
    • 1
  • Yulia V. Gerasimova
    • 2
  1. 1.University of Central Florida Department of ChemistryOrlandoUSA
  2. 2.University of Central Florida Department of ChemistryOrlandoUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-534-7
  • Online ISBN 978-1-62703-535-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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