Mouse Models of Allergic Disease

Methods and Protocols

  • Irving C.¬†Allen

Part of the Methods in Molecular Biology book series (MIMB, volume 1032)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Jennifer Skelton, Linda H. Gower, Anne McGough, Patricia A. Labosky
    Pages 1-17
  3. M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, Rita Carsetti
    Pages 45-57
  4. Kimberly D. Dyer, Katia E. Garcia-Crespo, Caroline M. Percopo, Eva M. Sturm, Helene F. Rosenberg
    Pages 59-77
  5. Amjad Horani, John D. Dickinson, Steven L. Brody
    Pages 91-107
  6. Jamie L. Sturgill, Daniel H. Conrad
    Pages 121-131
  7. Elizabeth Doyle, Julia Trosien, Martin Metz
    Pages 133-138
  8. Irving C. Allen
    Pages 139-144
  9. Virginia McMillan Carr, Alan M. Robinson
    Pages 145-158
  10. Monique A. M. Willart, Philippe Poulliot, Bart N. Lambrecht, Mirjam Kool
    Pages 185-204
  11. Sanchaita S. Sonar, Jan C. Dudda
    Pages 229-234
  12. Jeremiah T. Herlihy, Iurii Semenov, Robert Brenner
    Pages 257-269
  13. Back Matter
    Pages 323-326

About this book


Over the last half century, a dramatic increase in allergic diseases has been observed throughout industrialized nations, which has resulted in significant worldwide socio-economic challenges. In Mouse Models of Allergic Disease: Methods and Protocols, a wide range of expert contributors provide detailed protocols for the design and execution of experiments to thoroughly analyze critical elements associated with a diverse range of allergic diseases, all through the lens of mouse models that accurately recapitulate clinically relevant aspects of the respective human disease.  The volume opens with a section featuring techniques essential for effective ex vivo cell isolation and evaluation of specific cell types relevant to a diverse range of allergic diseases, and the book then moves on to cover in vivo protocols to evaluate prevalent mouse models of human allergic diseases, including mouse models of systemic anaphylaxis, contact hypersensitivity, allergic rhinitis, and asthma, as well as a collection of chapters on in vivo and ex vivo protocols used to assess indirect mediators of allergic diseases, such as the nervous system, non-hematopoietic cells, and the composition of the gut microbiome.  Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Timely and authoritative, Mouse Models of Allergic Disease: Methods and Protocols serves as an essential collection of protocols that allow both novice and expert researchers the ability to accurately develop, evaluate, and characterize the mechanisms associated with these disorders.


Allergic rhinitis Allergy Asthma Contact hypersensitivity Human allergic diseases In vivo and Ex vivo protocols Indirect mediators of allergies Mouse models Systemic anaphylaxis

Editors and affiliations

  • Irving C.¬†Allen
    • 1
  1. 1.Dept. of Biomedical Sciences & PathobiolVirginia Polytechnic Institute and State UniversityBlacksburgUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-495-1
  • Online ISBN 978-1-62703-496-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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