Membrane Biogenesis

Methods and Protocols

  • Doron Rapaport
  • Johannes M. Herrmann

Part of the Methods in Molecular Biology book series (MIMB, volume 1033)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Investigation of Membrane Lipids

    1. Front Matter
      Pages 1-1
    2. Cagakan Özbalci, Timo Sachsenheimer, Britta Brügger
      Pages 3-20
    3. Rania M. Deranieh, Amit S. Joshi, Miriam L. Greenberg
      Pages 21-27
  3. Analysis of Structure and Topology of Membrane Proteins

    1. Front Matter
      Pages 45-45
    2. Ian Collinson, Janet Vonck, Dilem Hizlan
      Pages 47-65
    3. Florian G. Müller, C. Roy D. Lancaster
      Pages 67-83
    4. Kristyna Pluhackova, Tsjerk A. Wassenaar, Rainer A. Böckmann
      Pages 85-101
    5. Christine T. Schwall, Nathan N. Alder
      Pages 103-120
    6. Iris Nasie, Sonia Steiner-Mordoch, Shimon Schuldiner
      Pages 121-130
    7. Katharina Wiesemann, Lucia E. Groß, Manuel Sommer, Enrico Schleiff, Maik S. Sommer
      Pages 131-144
  4. Studying Protein-Protein and Protein-Lipids Interactions within Membranes

    1. Front Matter
      Pages 145-145
    2. Zht Cheng Wu, Jeanine de Keyzer, Ilja Kusters, Arnold J. M. Driessen
      Pages 157-172
    3. Ron Saar-Dover, Avraham Ashkenazi, Yechiel Shai
      Pages 173-183
    4. Joseph D. Unsay, Ana J. García-Sáez
      Pages 185-205
    5. Takuya Shiota, Shuh-ichi Nishikawa, Toshiya Endo
      Pages 207-217
    6. Aline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault, Christine Ebel
      Pages 219-251
    7. Carolyn Vargas, Johannes Klingler, Sandro Keller
      Pages 253-271
  5. Membrane Biogenesis and Dynamics

    1. Front Matter
      Pages 273-273
    2. Dirk Scholz, Johannes Förtsch, Stefan Böckler, Till Klecker, Benedikt Westermann
      Pages 275-283
    3. Johanna Dudek, Sven Lang, Stefan Schorr, Johannes Linxweiler, Markus Greiner, Richard Zimmermann
      Pages 285-299
    4. Katrin Krumpe, Doron Rapaport
      Pages 301-306
    5. Daniel Weckbecker, Johannes M. Herrmann
      Pages 307-322
  6. Functional Analysis of Membrane Proteins

    1. Front Matter
      Pages 323-323
    2. Martin van der Laan, Ralf M. Zerbes, Chris van der Does
      Pages 325-344
    3. Philipp Bartsch, Anke Harsman, Richard Wagner
      Pages 345-361
    4. Juliana Heidler, Valentina Strecker, Florian Csintalan, Lea Bleier, Ilka Wittig
      Pages 363-379
    5. Anna Hjelm, Susan Schlegel, Thomas Baumgarten, Mirjam Klepsch, David Wickström, David Drew et al.
      Pages 381-400
  7. Back Matter
    Pages 401-404

About this book


Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques.  In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.


Biogenesis Cellular membranes Membrane lipids Membrane proteins Organelle dynamics Protein-Lipid interaction

Editors and affiliations

  • Doron Rapaport
    • 1
  • Johannes M. Herrmann
    • 2
  1. 1.Interfaculty Institute of BiochemistryUniversity of TuebingenTuebingenGermany
  2. 2.Cell BiologyUniversity of KaiserslauternKaiserslauternGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-486-9
  • Online ISBN 978-1-62703-487-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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